Hypolipidemic properties of bauhinia rufescens in alloxan-induced diabetic rats
Keywords:
Bauhinia rufescens, Lipid profile, Diabetic rats, AtheroslerosisAbstract
The present investigation was aimed at evaluating the hypolipidemic properties of methanolic leaf extracts of Bauhinia rufescens (MLEBR) on alloxan-induced diabetic rats. Alloxan was injected intraperitoneally as a single dose of 150mg/kg for diabetes induction in the rats. Animals were orally treated for 4 consecutive weeks with MLEBR at doses of 200, 300 400mg/kg and Glibenclamide. The effects of the extracts and Glibenclamide on lipid profile and body weight (BW) were examined on the diabetic rats. Total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), very low density lipoprotein cholesterol (VLD-C) and triglycerides (TG) levels at sacrifice (day 29) were estimated. Normal and Diabetic rats that were given normal saline only were used for comparison. Alloxan-induced diabetic rats showed moderate to significant increases in the levels of TC, LDL-C, VLDL-C and TG while body weight and HDL-C levels decreased compared to controls (non diabetic rats). Administration of the plant extracts to Alloxan-induced diabetic rats resulted in a significant decrease in TC, LDL-C, VLDL-C and TG and the dose 200 mg/kg of the MLEBR was the most effective; HDL-C level was markedly increased after four weeks post treatment compared to untreated diabetic rats. It can also be noticed that the MLEBR, especially the dose 200 mg/kg (p<0.01), produced more effects than glibenclamide. Rats treated with glibenclamide (5mg/kg) generally gave lower results compared to groups treated with the plant extracts. Results of the present study showed that methanolic leaf extracts of Bauhinia rufescens has beneficial effects on diabetic hyperlipidemia as such could be advanced in preventing the development of atherosclerosis and possible related cardiovascular pathologies associated with diabetes.
References
Aguh, I.B., Nock, I.H., Ndams, I.S., Agunu, A., 2013. Hypoglycaemic activity and Nephro-protective effect of Bauhinia rufescens in alloxan-induced diabetic rats. IJAPBC, 2(1), 249-255.
Aliyu, A.B., Ibrahim, M.A., Musa, A.M., Ibrahim, H., Abdulkadir, H.E., Oyewale, A.O., 2009. Evaluation of antioxidant activity of leave extract of Bauhinia rufescens Lam. (Caesalpiniaceae). J. Med. Plant. Res., 3(8), pp. 563-567.
American Diabetes Association, 2007. Diagnosis and classification of diabetes mellitus. Diabetes Care, 30: S42-S47.
Austin, M.A., Hokanson, J.E., 1994. Epidemiology of triglyceride, small dense low density lipoprotein and lipoprotein as risk factors for coronary heart disease. Med. Clin. North Am., 78, 99-115
Beckman, J.A., Creager, M.A., Libby, P., 2002. Diabetes and atherosclerosis: Epidemiology, pathophysiology and management. JAMA, 287, 2570-81.
Brown, M.S., Goldstein, J.L., 1983. Lipoprotein receptor in the liver. Control signal for plasma cholesterol traffic. J. Clin. Invest.,72, 743-7.
Burkill H. M., 1995. The useful plants of West Tropical Africa. vol. 3, 857 pp
Claudia, E.N.M., Julius, E.O., Dagobert, T., Etienne, D., 2006. Antidiabetic and hypolipidemic effects of Laportea ovalifolia (Urticaceae) in alloxan-induced diabetic rats. Afr. J. Trad. Complemen. Alter. Med., 3(1), 36-43.
Friedewald, W.T., Levy, R., Fradrickson, D.S., 1972. Estimation of concentration of low density lipoprotein cholesterol in plasma without the use of preparative ultracentrifuge. Clin. Chem.,19, 449-452.
Ganong, W.F. (2003). Review of medical physiology, 21st edition eBook-EEn. McGraw-Hill Company, Inc.
Iweala, E.E.J., Oludare, F.D., 2011. Hypoglycemic effect, biochemical and histological changes of Spondias mombin Linn.andParinari polyandra benth.Seed ethanolic extractsin alloxan-induced diabetic rats. J. pharmacol.Toxicol., 6, 101-112.
James, D.B., Owolabi, O.A., Ibrahim, A.B., Folorunsho, D.F., Bwalla, I., Akanta, F., 2010. Changes in lipid profile of aqueous and Ethanolic extract of Blighia sapida in rats. Asian J. Med. Sci.. Maxiwell Scientific Org.
Krishnakumar, K., Augustti, K.T., Vijayammal, P.L., 2000. Hypolipidemic effect of Salacia oblonga wall root bark in streptozotocin diabetic rats. Med. Sci., 28, 65-67.
Kumarappan, C. T., Mandal, S. C., 2007. Polyphenolic extract Ichnocarpus frutescens attenuates diabetic complications in streptozocin treated diabetic rats. Renal failure, 30(3): 307-22.
Kwiterovich, P.O., 2000. The metabolic pathways of high-density lipoprotein, low density lipoprotein and triglycerides: A current review. Ameri. J. Cardiol., 86(12), 5-10.
Mironova, M., Klein, R., Virella, G., Lopes-virella, M., 2000. Anti-modified LDL antibodies, LDL-containing immune complexes and susceptibility of LDL to in vitrooxidation in patients with type 2 diabetes. Diabetes, 49, 1033-104.
Momo, C.E.N., Oben, J.E., Tazoo, D., Dongo, E. 2006. Antidiabetic and hypolipemic effects of Laportea ovalifolia (Urticaceae) in alloxan induced diabetic rats. Afr. J. Trad. CAM, 3, 36-43.
Nimenibo-uadia, R., 2003. Effect of aqueous extract of Canavalia ensiformis seeds on hyperlipidemic and hyperkotonaemia in alloxan-induced diabetic rats. Biokemistri. ,15, 7-15.
Njike, G.N., Watco, P., Nguelefack, T.B., Kamanya, A., 2005. Hypoglycaemic activity of the leaves extracts of Bersama engleriana in rats. Afr. J. Trad., 2(3), 215-221.
Odetola, A.A., Akinloye, O., Egunjobi, C., Adekunle, W.A., Ayoola, A.O., 2006. Possible antidiabetic and antihyperlipidemic effect offermented Parkia Biglobosa (JACQ) extract in alloxan-induced diabetic rats. Clin. Exp. Pharmacol. Physiol., 33, 808-812.
Richard, A.H., Pamela, C.C., 2009. Lippincott’s illustrated Reviews: Pharmacology (4thed.) Lippincott Williams and Wilkin Wolters Kluwer Company. Baltimore., 249-295.
Richmond, N., 1973. Clin. Chem., 19, 1350-1356.
Roeschlau, P., Bernt, E., Gruber, J. W., 1974. Clinic. Chem. Biochem., 12, 403.
Rotimi, S.O., Omotosho, O.E., Roimi, O.A., 2011. Persistence of acidosis in alloxan induced diabetic rats treated with the juice of Asystasia gangetica leaves. Phcog. Mag., 7, 25-30.
Saunders Comprehensive Veterinary Dictionary, 3 ed. © 2007 Elsevier, Inc.
Sharma, S.B., 2003. Hypoglycemic and hypolipidemic effect of ethanolic extract of seed of Eugenia jambolana in alloxan-induced diabetic rabbits. J. Ethnopharmacol., 85, 201-206.
Sobngwi, T., Mauvais-jarvis, F., Vexiau, P., Gautier, J.F., 2001. Diabetes in Africans Epidemiology and clinical specificities. Diabetes Metab. (Paris), 27, 628-34.
Subbiah, R., Kasiappan, R., Karuran, S., Sorimuthu, S., 2006. Beneficial effects of Aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes. Clin. Exp. Physiol., 33, 232-237.
Suryawanshi, N.P., Bhutey, A.K., Nagdeote, A.N., Jadhav, A.A., Manoorkar, G.S., 2006. Study of lipid peroxide and lipid profile in diabetes mellitus. Indian J. Clin. Bioch. (1), 126-130.
Tietz, N.W., 1990. Cinical Guide to Laboratory Tests, Second EditionW. B. Sanders Company, Philadelphia, USA, 554-556.
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Copyright (c) 2013 Bruno Ikenna Aguh, Ishaya Haruna Nock, Iliya Stanley Ndams, Abdulkarim Agunu, Patrick Oluwagbemiga Ayeku
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