Potentially pathogenic environmental strains of non-tuberculous mycobacteria from wastewater samples from the municipalities of Yopougon and Koumassi-Marcory in Abidjan

Authors

  • Sabine N'dri Vakou Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan, Côte d’Ivoire.
  • Julien Coulibaly-Kalpy -Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan, Côte d’Ivoire. -University of Felix Houphouët Boigny, Ivory Coast, Côte d’Ivoire.
  • Koui Tossea -Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan, Côte d’Ivoire. -University of Felix Houphouët Boigny, Ivory Coast, Côte d’Ivoire.
  • Eric Kouamé Yao -Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan, Côte d’Ivoire. -University of Felix Houphouët Boigny, Ivory Coast, Côte d’Ivoire.
  • Mireille Dosso Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan, Côte d’Ivoire.
  • Allico J. Djaman -Pasteur Institute of Ivory Coast, 01 BP 490 Abidjan, Côte d’Ivoire. -University of Felix Houphouët Boigny, Ivory Coast, Côte d’Ivoire.

Keywords:

Mycobacteria, Mycolicibacterium, Mycobacteriosis, Buruli ulcer, Tuberculosis

Abstract

Mycobacteria represent a large group of bacteria commonly found in the environment. They are involved in several infections ranging from lung infections to skin infections. In Côte d'Ivoire, very little information is available on these species apart from the best known, namely M. ulcerans and M. tuberculosis, responsible for Buruli ulcer and tuberculosis respectively. The cultivation of these species is a real challenge, especially in developing countries such as Côte d'Ivoire. However, there are reports in the literature of infections caused by these mycobacteria and few species have been described in cases of human or animal infections. Mycobacteriosis due to these mycobacteria is difficult to estimate because these diseases are not reportable illness. These pathologies are difficult to treat because of their resistance to most anti-tuberculosis antibiotics. The aim of our study was to identify the strains of potentially pathogenic non-ulcer and non-tuberculosis environmental mycobacteria circulating in the wastewater in the city of Abidjan. The strains isolated in this study were fast-growing mycobacteria and slow-growing mycobacteria. Thanks to the sequencing of the amplification product, 5 species of mycobacteria were identified, namely mycolicibacterium fortuitum; mycolicibacterium mageritense; mycolicibacterium europaeum; mycolicibacterium neworleansense and mycolicibacterium Brumae. This study would be the first to identify these fast-growing and slow-growing species in Côte d'Ivoire.

References

Aka, N.G., Coulibaly, N.D., Kouamé Elogne, N.C., Acquah, K.J.R., Amon, A.C., Kouadio, K., Konan, N.G., Koffi, A., Kadio, M.C., Yao, A., Saunderson, P., Kakou-Ngazoa, E.S., Faye-Ketté, H et Dosso, M., 2018. Phenotypic and genotypic characterization of mycobacteria isolates from Buruli Ulcer suspected patients reveals the involvement of several mycobacteria in chronic skin lesions. Am. J. Microbiol. Res., 6(3), 79-87.

Aka, N.G., Kakou, S., Coulibaly, N.D., Vakou, S., Kouadio, K., Aoussi, S., Kadio, M.C., Kouakou, H., Ake, J., N’guessan, R., Dosso, M., 2015. Virulence factors expressed by Mycobacterium ulcerans strains: Results of a descriptive study. Afr. J. Microbiol. Res., 9, 1745-1751.

Al Jarad, N., Demertzis, P., Meecham Jones, D.J., Barnes, N.C., Rudd, R.M., Gaya, H., Wedzicha, J.A., Hughes, D.T., Empey, D.W., 1996. Comparison of characteristics of patients and treatment outcome for pulmonary nontuberculous mycobacterial infection and pulmonary tuberculosis. Thorax, 51(2), 137-139.

Asmar, S., Robert, C., Croce, O., Caputo, A., Drancourt, M., 2015. Draft genome sequence of Mycobacterium neworleansense strain ATCC 49404T. Genome Announc., 3, e01314-15.

Azadi, D., Shojaei, H., Pourchangiz, M., Dibaj, R., Davarpanah, M., Naser, A.D., 2016. Species diversity and molecular characterization of nontuberculous mycobacteria in hospital water system of a developing country, Iran. Microb. Pathog., 100(100), 62-69.

Barksdale, L., Kim, K.S., 1977. Mycobacterium. Bacteriol. Rev., 41, 217.

Britton, L.J., Gresson, P.E., 1987. Methods for collection and analysis of aquatic biological and microbiological samples. Chapter A4 in techniques of water resources investigations of the United States Geological Survey, USGS, 79, 1279, 1979.

Brown-Elliott, B.A., Wallace, RJ Jr., 2002. Clinical and taxonomic status of pathogenic nonpigmented or late-pigmenting rapidly growing mycobacteria. Clin. Microbiol. Rev., 15, 716-746.

Clarridge, J.E., 2004. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin. Microbiol. Rev., 17, 840-862.

Clovice, K., Adrian, M., Berit, D., Musso, M., John, O-A., Eystein, S., James, O., Vigdis, E., Tone, B.J., 2011. Isolation of non-tuberculous mycobacteria from pastoral ecosystems of Uganda: Public Health significance. BMC Publ. Health, 11(1), 1471-2458.

Domenech, P., Jimenez, M.S., Menendez, M.C., Bull, T.J., Samper, S., Manrique, A., 1997. Mycobacterium mageritense sp. Int. J. Syst. Bacteriol., 47, 535-540.

Euzéby, J.P., 2010. Techniques de diagnostiques en mycobactériologie. Dictionnaire de Bactériologie Vétérinaire.

Gazeroglu, I., Borenstein, M., Solano, M.P., 2004. Case study: skin infection in a diabetic patient related to contamination of an insulin bottle. Clin. Diabet., 22, 137-145.

Gira, A.K., Reisenauer, A.H., Hammock, L., Nadiminti, U., Macy, J.T., Reeves, A., 2004. Furunculosis due to Mycobacterium mageritense associated with footbaths at a nail salon. J. Clin. Microbiol., 42, 1813-1817.

Gomez-Moyano, E., Del Boz Gonzalez, J., Bermudez-Ruiz, P., Sanz-Trelles, A., 2009. Furuncle-like lesions by Mycobacterium mucogenicum in a immunocompetent patient. Med. Clin. (Barc), 132, 370.

Gupta, R.S., Lo, B., Son, J., 2018. Phylogenomics and comparative genomic studies robustly support division of the genus mycobacterium into an emended genus mycobacterium and four novel genera. Front. Microbiol., 9, 67.

Hartsman, S.A.M., Debont, J., Stackbrandt, E., 2006. The genus mycobacterium- no medical. Prokaryotes, 3, 889-918.

Hoefsloot, W., van Ingen, J., Andrejak, C., Angeby, K., Bauriaud, R., Bemer, P., 2013. The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: an NTM-NET collaborative study. Eur. Respir. J., 42, 1604-1613.

Ishii, N., Sugita, Y., Sato, I., Nakajima, H., 1998. A case of mycobacterial skin disease caused by Mycobacterium peregrinum and M. scrofulaceum. Acta. Derm. Venereol., 78, 76-77.

ISO: Qualité de l'eau - Échantillonnage - Partie 15: Lignes directrices pour la conservation et le traitement des échantillons de boues et de sédiments. ISO. 2009, 5667-15.

Janda, J.M., et Abbott, S.L., 2007. 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls. J. Clin. Microbiol., 45, 2761-2764.

Katoch, V.M., Parashar, D., Chauhan, D.S., Singh, D., Sharma, V.D., Gosh, S., 2007. Rapid

identification of mycobacteria by gene amplification restriction analysis technique targeting

S-23S ribosomal RNA internal transcribed spacer & flanking region. Indian J.

Med. Respir., 155-162.

Kothavade, R.J., Dhurat, R.S., Mishra, S.N., Kothavade, U.R., 2013. Clinical and laboratory aspects of the diagnosis and management of cutaneous and subcutaneous infections caused by rapidly growing mycobacteria. Eur. J. Clin. Microbiol. Infect. Dis., 32(2), 161-88.

Laurence, A., Aka, N., Euloge, E., Samir, B., Raymond, N., Mireille, D., 2009. Risk factors for Buruli ulcer in Côte d’Ivoire: Results of a case-control study. Afr. J. Biotech., 8(4), 536-546, 18.

Lee, M.R., Sheng, W.H., Hung, C.C., Yu, C.J., Lee, L.N., 2015. Mycobacterium abscessus complex infections in humans. Emerg. Infect. Dis., 21, 1638.

Luquin, M., Ausina, V., Vincent-Levy-Frebault, V., Laneelle, M.A., Belda, F., Garcia-Barcelo, M., Prats, G., Daffe, M., 1993. Mycobacterium brumae sp. nov., a rapidly growing, nonphotochromogenic mycobacterium. Int. J. Syst. Bacteriol., 405-413.

Metchock B Nolte, F., 1995. Mycobacterium. In: Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C., Yolken, R.H. (eds.): Manual of Clinical Microbiology, 6th ed. American Society for Microbiology, Washington, DC. 400-437.

Oren, A., Trujillo, M.E., 2019. On the valid publication of names of mycobacteria. Eur. Respir. J., 54(4), 1901483.

Park, I.K., Olivier, K.N., 2015. Nontuberculous mycobacteria in cystic fibrosis and non-cystic fibrosis bronchiectasis. Semin. Respir. Crit. Care Med., 36, 217-224.

Phelippeau, M., Croce, O., Robert, C., Raoult, D., Drancourt, M., 2015. Draft genome sequence of Mycobacterium Europaeum strain CSUR P1344. Genome Announc., 3(4), e00816-15.

Phillips, M.S., von Reyn, C.F., 2001. Nosocomial infections due to nontuberculous mycobacteria. Clin. Infect. Dis., 33, 1363-1374.

Pinner, M., 1935. Atypical acid fast microorganisms. Am. Rev. Tuberc., 32, 424-45.

Portaels, F., Fonteyene, P.A., de Beenhouwer, H., de Rijk, P., Guedenon, A., Hayman, J., et Meyers, M.W., 1996. Variability in 3' end of 16S rRNA sequence of Mycobacterium ulcerans is related to geographic origin of isolates. J. Clin. Microbiol., 34, 962-965.

Pourahmad, F., Shojaei, H., Heidarieh, P., Khosravi, A., Hashemi, A., 2012. Report of two cases of Mycobacterium Europaeum from Iran. Jpn. J. Infect. Dis., 65, 539-541.

Réunion de l’OMS sur l’ulcère de Buruli et autres maladies tropicales négligées à manifestation cutanée. Rapport final, 25 au 27 mars 2019, Siège de l’OMS Genève, Suisse.

Rodriguez, J.A., Bonnano, C., Khatiwada, P., Roa, A.A., Mayer, D., Eckardt, P.A., 2021. COVID-19 coinfection with Mycobacterium abscessus in a patient with multiple Myeloma. Hindawi Case Reports in Infectious Diseases Volume 2021, Article ID 8840536, 5 pages https://doi.org/10.1155/2021/8840536

Runyon, E.H., 1959. Anonymous mycobacteria in pulmonary disease. Med. Clin. N. Am., 43, 273-290.

Schinsky, M.F., Morey, R.E., Steigerwalt, A.G., 2004. Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp nov, Mycobacterium houstonense sp nov, Mycobacterium neworleansense sp nov and Mycobacterium brisbanense sp nov and recognition of Mycobacterium porcinum from human clinical isolates. Int. J. Syst. Evol. Microbiol., 54, 1653-1667.

Shinnick, T.M., Good, R.C., 1994. Mycobacterial taxonomy. Eur. J. Clin. Microbiol. Infect. Dis., 13, 884-901.

Soro, N., Ouattara, L., Dongo, K., Kouadio, E.K., Ahoussi, E.K., Soro, G., Oga, M.S., Savane, I., Biemi, et J., 2010. Déchets municipaux dans le District d’Abidjan en Côte d’Ivoire: sources potentielles de pollution des eaux souterraines. Int. J. Biol. Chem. Sci., 4(6), 2203-2219.

Stinear, T., Ford, T., Vincent, V., 2004. Analytical methods for the detection of waterborne and environmental pathogenic mycobacteria. 55-73. In: Pedley, S., Bartram, J., Rees, Dufour, A., Cotruvo, J. (ed.), Pathogenic mycobacteria in water: a guide to public health consequences, monitoring and management. IWA Publishing, London, United Kingdom.

Tortoli, E., Böttger, E.C., Fabio, A., Falsen, E., Gitti, Z., Grottola, A., Klenk, H.P., Mannino, R., Mariottini, A., Messinò, M., Pecorari, M., Rumpianesi, F., 2011. Mycobacterium europaeum sp. nov., a scotochromogenic species related to the Mycobacterium simiae complex. Int. J. Syst. Evol. Microbiol., 61, 1606-1611.

Tortoli, E., Brown-Elliott, B.A., Chalmers, J.D., Cirillo, D.M., Daley, C.L., Emler, S., Floto, R.A., Garcia, M.J., Hoefsloot, W., Won-Jung, K., Lange, C., Loebinger, M., 2019. Same meat, different gravy: ignore the new names of mycobacteria. Eur. Respir. J., 54(1), 1900795.

Van Ingen, J., Boeree, M.J., Dekhuijzen, P.N., van Soolingen, D., 2009. Environmental sources of rapid growing nontuberculous mycobacteria causing disease in humans. Clin. Microbiol. Infect., 15, 888-893.

Varghese, B., Enani, M., Shoukri, M., AlThawadi, S., AlJohani, S., Al- Hajoj, S., 2017. Emergence of rare species of nontuberculous mycobacteria as potential pathogens in Saudi Arabian Clinical Setting. PloS. Negl. Trop. Dis., 11(1), e0005288.

Wallace Jr, R.J., Brown, B.A., Griffith, D.E., 1998. Nosocomial outbreaks/pseudooutbreaks caused by nontuberculous mycobacteria. Annu. Rev. Microbiol., 52, 453-490.

Wallace Jr, R.J., Brown-Elliott, B.A., Hall, L., Roberts, G., Wilson, R.W., Mann, L.B., 2002. Clinical and laboratory features of Mycobacterium mageritense. J. Clin. Microbiol., 40, 2930-2935.

Wilton, S., Cousins, D., 1992. Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube. PCR Method. Appl., 1, 269-273.

Published

2022-01-29

How to Cite

Vakou, S. N., Coulibaly-Kalpy, J. ., Tossea, K. ., Kouamé Yao, E. ., Dosso, M. ., & Djaman, A. J. . (2022). Potentially pathogenic environmental strains of non-tuberculous mycobacteria from wastewater samples from the municipalities of Yopougon and Koumassi-Marcory in Abidjan. Scientific Journal of Microbiology, 8(1), 188-196. Retrieved from http://sjournals.com/index.php/sjm/article/view/1683

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