Molecular characterization of potential allergenic molds (Aspergillus, Penicillium and Fusarium) in two buildings receiving the public in Abidjan – Côte d’Ivoire
Keywords:
Allergy, Moulds, Aspergillus, Penicillium, Fusarium, PCR.Abstract
Aspergillus, Penicillium and Fusarium species play an important role in environmental allergy. These molds are potential density in the indoor environment. This study aimed to characterize the strains Aspergillus, Penicillium and Fusarium using a molecular method. The beta-tubulin and elongation factor TEF- genes were utilized to differentiate the species. A total of 405 environmental species (Aspergillus, Penicillium and Fusarium) were isolated, purified and initially identified by colony morphology. Subsequently, DNA was extracted and; PCR was performed from 351 isolates. The results were then compared to morphological characteristics. Of the 351 isolates tested, 167 were Aspergillus, 103 were Penicillium and 81 were Fusarium. The beta-tubulin and elongation factor TEF genes were found to be the most suitable for differentiating these three genera among them; the beta-tubulin gene was used for molecular identification of Aspergillus, Penicillium and the elongation factor TEF gene for characterizing Fusarium species.
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Copyright (c) 2021 M’boh Epi Reine Elisabeth N’Gou, David Coulibaly N’Golo, Chiaye Claire Antoinette Yapo-crezoit, Ama Valerie Bonouman, Solange Kakou, Ossey Bernard Yapo, Allico Joseph Djaman, Mireille Dosso
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