Isolation, identification and characterization of Clostridium perfringens from lamb dysentery in Dinajpur district of Bangladesh

Abstract

The present study was conducted for isolation, identification and characterization of Clostridium perfringens organism from lamb dysentery in Dinajpur district of Bangladesh. Affected sheep showed clinical signs as dysentery, diarrhea, anorexia, weakness, and dehydration. A total of 20 faeces and rectal swab were collected from different sheep farms of different area of Dinajpur District of Bangladesh. Faeces and rectal samples were collected aseptically and microbial examination was done by using Gram’s staining, cultural and biochemical techniques in the Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh. Out of 20 faeces and rectal swab samples 5 were found to be positive. The percentages of positive sample were 25%, 40%, 12.5% and 33.33% respectively in Rezanur sheep farm, Khalilur sheep farm, Hasan sheep farm and Mojammal sheep farm.  Antimicrobial susceptibility test was performed by discs diffusion method. Most of the isolated Clostridium perfringens were susceptible to ciprofloxacin, levofloxacin and penicillin. However, most of the isolated Clostridium perfringens were resistant to gentamycin, streptomycin and chloramphenicol. The antimicrobial susceptibility testing reaveled that penicillin, ciprofloxacin and levofloxacin were most efficacious followed by amoxycillin and azithromycin. So, it may be recommended that penicillin, levofloxacin and ciprofloxacin in optimum doses would resolve most cases of lamb dysentery in the Dinajpur district of Bangladesh.