In vitro plant regeneration, screening for salt and drought tolerance in sugarcane (Saccharum Spp.)
Keywords:
Calli, Root regenerants, Shoots regenerants, Selection, Tissue culture, VariabilityAbstract
Soil salinity and drought are some of the major sugarcane production factors in Ethiopia. This investigation was conducted to develop, screen and select salt and drought tolerant genotypes under in vitro condition. Both direct and indirect pathways were employed in the development and selection of salt and drought tolerant lines from the commercial varieties. In all cultures, explants were inoculated to Murashige and Skoog (MS) medium supplemented with inhibitory levels of NaCl (0, 30, 100, 170 & 240 mM) and polyethylene glycol (0, 2.5, 5, 7.5 & 10%) concentrations. During callus induction, data for percent of explants induced callus and its morphological status were collected to evaluate varieties, and select healthy, salt and polyethylene glycol (PEG) tolerant calli. Moreover, shoot regeneration percentage, shoot Vigrousity and color were taken as major criteria to select stress (salt and PEG) tolerant shoot while data for plantlet root attributes were collected to screen stress tolerant plantlets. Results of the study indicated that health and salt tolerant calli and shoots were identified at MS medium supplemented with 170 Mm while 10% PEG was the best selection medium to select healthy and PEG tolerant calli and shoots. Our screening results suggested that the in vitro developed regenerants were more discriminated at 240 mM NaCl where S4SP-70-1284, S4 C0-501 and S2B-78-505 were selected as salt tolerant regenerants. Moreover, more variability was observed at MS medium induced with 10% polyethylene glycol where regenerants D4C-132-81, D5C-132-81, D3B52-298, D2B52-298 and D4B52-298 showed better degree of tolerance to drought. The dendrogram separated those regenerants developed through direct regeneration from those regenerants developed through indirect regeneration. It suggested the existence of somaclonal variation. Moreover, both dendrograms were capable of separating those regenerants exhibiting tolerance to the stresses in question thereby suggesting the existence of variability in relation to stress tolerance. We recommend these regenerants be used as a source of variability for future stress breeding studies. Moreover, these regenerants can be used as alternative varieties at salt affected and soil moisture limited fields of sugarcane production areas. However, it should be further evaluated for yield and yield quality before using for commercial purposes.
References
Arzani, A., 2008. Improving salinity tolerance in crop plants: A biotechnological view. In Vitro Cell Development Biololgy Plant, 44, 373-383. Ashok, K., Shrivastava, A., Srivastava, K., Solomon, S., 2011. Sustaining sugarcane productivity under depleting water resources. Curr. Sci., 101, 748-754. Ashraf, M., Rahmatullah, K.S., Tahi, M.A., Sarwar, A., Ali, L., 2007. Differential salt tolerance of sugarcane genotytpes. Directorate of Land Reclamation, Irrigation & Power Department, Punjab, Pakistan. Pakistan J. Agr. Sci., 44, 85-89. Ather, A., Khan, S., Rehman, A., Nazir, M., 2009. Optimization of the protocol for callus induction, regeneration and acclimatization of sugarcane. CV. Thatta- 10. Pakistan J. Botany, 41(2), 815-820. Avinash, L., Prashant, N., Bharose, A.A., 2012. In vitro screening of sugarcane cultivar Co 86032 for salinity tolerance. College of Agricultural Biotechnology Marathwada Krishi Vidyapeeth Latur, India. J. Sugar Res., 2, 25-31. Begum, M.K., Islam, M.O., Miah, M.A.S., Hossain, M.A., Islam, N., 2011. Production of somaclone: In vitro for drought stress tolerant plantlet selection in sugarcane (Saccharum officinarum L.). Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna, Bangladesh. Agr., 9, 18-28. Begum, M.S., Kohinoor, T., Islam, M.D.O., 2015. Selection of salt tolerant somaclones for development of salt stress tolerant varieties. Plant, 3, 37-46. Biradar, S., Biradar, D.P., Patil, V.C., Patil, S.S., Kambar, N.S., 2009. In vitro plant regeneration using shoot tip culture in commercial cultivar of sugarcane. Karnataka J. Agr. Sci., 22, 21-24. Cha-um, S., Chuencharoen, S., Mongkolsiriwatana, C., Ashraf, M., Kirdmanee, C., 2012. Screening sugarcane (Saccharum sp.) genotypes for salt tolerance using multivariate cluster analysis. National Center for Genetic Engineering and Biotecnology. Plant Cell, Tissue Organ Culture, 110, 23-33. Gadakh, S.S., Patel, D.U., Patil, A.B., 2015. Evaluation of sugarcane (Saccharum officinarum L.) mutants for yield, yield contributing traits and quality paramaters. Department of Genetics & Plant Breeding, N.M. College of Agriculture, Navsari Agricultural University, Navsari. I.J.A.B.R., 5, 220-228. Gandonou, C.H., Errabini, T., Abrinii, J., Idaomar, M., Chibi, F., Skali, S.N., 2005. Effect of genotype on callus induction and plant regeneration from leaf explants of sugarcane. Afr. J. Biotechnol., 4, 1250-1255. Garcia, A.A., Kido, E.A., Meza, A.N., Souza, H.M.B., Pinto, L.R., Pastina, M.M., Leite, C.S., Silva, D., Ulian, E.D., Figueira, A., Souza, A.P., 2006. Development of an integrated genetic map of a sugarcane (Saccharum spp.) commercial cross; based on maximum likelihood approach for estimation of linkage and linkage phases. Theoret. Appl. Genet., 112, 298-314. Girma, A., 1993. Assessment of salinity effect and sodicity status of Metahara sugar estate. MSc Thesis. Alemaya University, College of Agriculture, Ethiopia. Gower, J.C., 1971. A general coefficient of similarity and some of its properties. Biometr., 27, 857-871. Hadi, M.Z., Bridgen, M.P., 1996. Somaclonal variation as a tool to develop pest resistant plants of Torenia fournieri ‘Compacta Blue’. Plant Cell, Tissue Organ Culture, 46, 43-50. Hemarparabha, G., Nagarajan, R., Alarmelu, S., 2004. Response of sugarcane genotypes to water deficiet stress. Sugar Technol., 6, 165-168. Islam, M.W., Miah, M.A.S., Hossain, M.A., Begum, M.K., Islam, M.S., 2010. Selection of drought stress tolerant somaclones of sugarcane under field condition. Int. J. Sustain. Agr. Technol., 6, 52-60. Kenganal, M., Hanchinal, R.R., Nadaf, H.L., 2008. Ethyl methane sulphonate (EMS) induced mutation and selection for salt tolerance in sugarcane in vitro. Indain J. Plant Physiol., 13, 405-410.
Khan, I.A., Dahot, M.U., Seema, N., Yasmin, S., Bibi, S., Raza, S., Khatri, A., 2009. Genetic variability in sugarcane plantlets developed through in vitro mutagenesis. Pakistan J. Bot., 4, 153-166. Larkin, P.J., Scowcroft, W.R., 1981. Somaclonal variation: A novel source of variability from cell cultures for plant improvement. Theoret. Appl. Genet., 60, 197-214. Maas, E.V., 1986. Salt tolerance in plants. Appl. Agr. Res., 1, 12-26. Mahmoud, S., Majid, N., Mosa, M., Hamid, R., 2011. Evaluation of sugarcane (Saccharum officinarum L.) somaclonals tolerance to salinity via in vitro and In vivo culture. Afr. J. Biotechnol., 10, 9337-9343. Megersa, O., Willibald, L., Josef, F., 2009. Effect of lake Basaka expansion on the sustainability of Matahara SE in the Awash River basin, Ethiopia. Water, sanitation and hygiene: Sustainable development and multisectoral approaches. 34th WEDC International Conference, Addis Ababa, Ethiopia. Munir, N., Aftab, F., 2013. Effect of salt stress on callus morphology and growth of sugarcane callus cultures. Department of Biotechnology, Lahore College for Women University, Lahore. Pakistan J. Sci., 65, 473-477. Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15, 473-497. Naidoo, S., Van, R.A., Parmmenter, N.W., 2004. Quantifying the effect of soil salinity on the physiology of three South African sugarcane varieties. Council for Scientific and Industrial Research, South Africa. Proceeding South African Sugar Technology Association, 78, 1-19. Patade, V.P., Suprasanna, P., 2009. An in vitro radiation induced mutagenesis selection system for salinity tolerance in sugarcane. Functional Plant Biology Section, Nuclear Agriculture and Biotechnology Division, Bhabha. Sugar Technol., 42-44. Patade, V.Y., Suprasanna, P., Bapat, V.A., Kulkarni, U.G., 2006. Selection for abiotic (salinity and drought) stress tolerance and molecular characterization of tolerant lines in sugarcane. Bhabha Atomic Research Centre Newsletters, 27, 244-257. Patel, S.R., 2007. Induction of variability through callus culture in sugarcane (Saccharum oficinarum L.) variety CoN 95132. Ph.D. Thesis submitted to N.A.U., Navsari. Perez-Clemente, R.M., Gomez-Cadenas, A., 2012. In vitro tissue culture, a tool for the study and breeding of plants subjected to abiotic stress conditions. Department of Agricultural Sciences. Universidad Jaume I, Spain. Recent Advances in Plant in Vitro Culture. Rai, M.K., Kalia, R.K., Singh, R., Gangola, M.P., Dhawan, A.K., 2011. Developing stress tolerant plants through in vitro selection: An overview of the recent progress. Experimental Botany, 71, 89-98. Rajeswari, S., Krishnamurthi, M., Shinisekar, S., Prem, A.S., Thirugnana, K.S., 2009. Performance of somaclones developed from intergeneric hybrids of sugarcane. Sugar Technol., 11, 258-261. Raza, G., Ali, K., Mukhtar, Z., Mansoor, S., Arshad, M., Asad, S., 2010. The response of sugarcane (Saccharum officinarum L.) genotypes to callus induction, regeneration and different concentrations of the selective agent (geneticin-418). Afr. J. Biotechnol., 9, 8739-8747. Rengasamy, P., 2010. Soil processes affecting crop production in salt affected soil. School of Agriculture, Food and Wine, University of Adelaide, Australia. Funct. Plant Biol., 37, 613-620. Rozeff, N., 1995. Sugarcane and Salinity: A review paper. Sugarcane, 5, 8-19. Sakhanokho, H.F., Kelley, R.Y., 2009. Influence of salicylic acid on in vitro propagation and salt tolerance in Hibiscus acetosella and Hibiscus moscheutos (cv ‘Luna Red’). Afr. J. Biotechnol., 8, 1474-1481. Shah, J.K., Khan, M.A., Ahmad, H.K., Khan, R.D., Zafar, Y., 2004. Somaclonal variation in sugarcane through tissue culture and subsequent screening for salt tolerance. Asian J. Plant Sci., 3, 330-334. Shomeili, M., Majid, N., Mosa, M., Rajabi, M., 2011. Evaluation of sugarcane (Saccharum officinarum L.) somaclonal varients tolerance to salinity in vitro and In vivo cultures. Afr. J. Biotech., 10, 9337-9343. Srinath, R., Jabeen, F.T.Z., 2013. In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.). Physiol. Mol. Biol. Plant, 19, 261-268. Statistical Analysis System Institute, 2009. SAS Users Guide; Version 9.2. SAS Ins. Inc. Cary North Carolina, USA. Steel, R.G.D., Torrie, J.H., 1981. In: Principles and procedures of statistics. McGraw Hill International Book Company, London (2nd Edition), 254-238. Subbarao, G.V., Johanssen, C., 1999. Strategies and scope for improving salinity tolerance in crop plants. In: Advanced Life Support Systems Group. John F. Kennedy Space Center Kennedy Space Center, Florida.
Subbarao, M., Shaw, M.A.E., 1985. A review of research on sugarcane soils of Jamaica. Proceeding of Meeting West Indies Sugar Technology, 2, 343-355. Tate, B., 2009. Re-evaluation of the plantation soils at Metahara sugar factory, Ethiopia. Vasantha, S., Venkataramana, P.N., Gururaja, R., Gomathi, R., 2009. Long term salinity effect on growth, photosynthesis and osmotic characteristics in sugarcane. Biol. Life Sci., 12, 5-8. Wagih, M.E., Aal, A., Musa, Y., 2003. Regeneration and evaluation of sugarcane somaclonal variants for drought tolerance. Sugarcane Technol., 2, 35-40.
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