Intestinal microbiota composition with probiotic potential of three species of the genus Chirostoma
Keywords:
Chirostoma, Microbiota, Intestinal tract, ProbioticsAbstract
The aim of this research was to identify the microbiota that was found in C, jordani, C. humboldtianum and C. estor to obtain those strains with probiotic capacity and can be used in Chirostoma sp. culture. Pre-adult stages were obtained for dissection and gain an intestinal tissue portion and get pure strains throughout consecutive reseeding in NHI and TSA agar plates. Then proceeded to the molecular identification with RNAr 16S sequencing gen and establish their probiotic capacity growing them in acid pH, bile salts, pathogen inhibition to Aeromonas hydrophila and Vibrio fluvialisin vitro and with antibiotics according to FAO (2006) and Balcázaret al. (2006) techniques. Results shown that analyzed Chirostoma sp. from intestinal tract were dominated by Proteobacteria, Actinobacteria and Firmicutes identifying 60 strains, 40% of them were from C. jordani; 30% from C. humboldtianum and 20% of strains were from C. estor. The three analyzed species share some bacterial groups even if they were grown in different environmental conditions. The common species strains were Lactobacillus sp., L.crispatus, Bacillus subtilis, B. sp., Aeromonashydrophila, A.hydrophila caviae, Vibrio fluvialis and Pseudomonaluteola. The only bacterial species that resisted stress experiments, correspond to Bacillus sp. genus represented by Bacillus subtilis, Bacillus sp. and Bacillus laterodporus, so they are good probiotics candidates for culture of Chirostoma sp. genus.References
Aguilera, E., Yany, G., Romero, J., 2013. Cultivable intestinal microbiota of yellowtail juveniles (Seriolalalandi) in an aquaculture system. Lat. Am. J. Aquat. Res., 41(3), 395-403.
Al-Harbi, A.H., Uddin, M.N., 2005. Bacterial diversity of tilapia (Oreochromisniloticus) cultured in brackish water in Saudi Arabia. Aquaculture., 250, 566-572.
Ávila, J., Ávila, M., Tovar, B., Brizuela, M., Perazzo, Y.yH. Hernández, 2010. Capacidad probiótica de cepas del género Lactobacillus extraídas del tracto intestinal de animales de granja. Rev. Científica., FCV-LUZ. 20(2), 161–169.
Balcázar, J., De Blas, I., Ruiz, I., Cunningham, D., Vendrell, D.yJ. Múzqui, 2006. The role of probiotics in aquaculture. Vet. Microbiol., 114, 173–186.
Befring, H.M., Tore, B.L., Fontanillasd, R., Rosnesa, J.T., 2007. Molecular characterisation of the intestinal microbiota of farmed Atlantic salmon (Salmo salar L.). Aquaculture., 272(1–4), 581–588.
Dopazo, P., Lemos, L., Lodeiros, C., Bolinches, J., Barja, L.yA. Toranzo, 1988. Inhibitory activity of antibiotic-producing marine bacteria against fish pathogens. J. Appl. Bacteriol., 65, 97–101.
Dunne, C., O’Mahony, L., Murphy, L., Thornton, G., Morrissey, D., O’Halloran, S., Feeney, M., Flynn, S., Fitzgerald, G., Daly, C., Kiely, B., O’Sullivan, G., Shanahan, F.yK. Collins, 2001. In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am. J. Clin. Nutr., 73, 386–392.
Elías–Fernández, G., Navarrete-Salgado, N.A., Rodríguez-Robles, J.L., 2008. Alimentación de Chirostoma humboldtianum (Valenciennes); (Pisces: atherinopsidae) en el estanque JC en Soyaniquilpan, Estado de México. Revista Chapingo. Serie Ciencias Forestales y del Ambiente, 129-134.
FAO, 2006. Probióticos en los alimentos propiedades saludables y nutricionales y directrices para la evaluación. Roma, Organización de las Naciones Unidas para la Agricultura y la Alimentación (FAO). 85. ftp://ftp.fao.org/docrep/fao/009/a0512s/a0512s00.pdf.
Ghosh, K., Sen, S.K., Ray, A.K., 2002. Characterization of bacilliisolated from the gut of rohu,Labeorohitafingerlings and itssignificance in digestion. J. Appl. Aquac., 12, 33–42.
Hamdan, P., 2004. Biomonitoreo: seguimiento de poblaciones microbianas en procesos de biorremediación de suelos contaminados con hidrocarburos. Tesis para obtener el grado de Maestro en biotecnología. México, Universidad Autónoma Metropolitana- Iztapalapa, 77.
Hooper, L., Midwedt, T., Gordon, J., 2002. How host microbial interactions shape the nutrient environment of the mammalian intestine. Ann. Rev. Nutr., 22, 283–307.
Jaffrès, E., Sohier, D., Leroi, F., Pilet, M.F., Prévost, H., Joffraud, J.J., y Dousset, X., 2010. Study of the bacterial ecosystem in tropical cooked and peeled shrimps using a polyphasic approach. Int. J. Food. Microbiol., 131, 20-29.
Lin, Y., Jon, A., Duane, C., Mark, G., Wen-Tso, L., 2014. Fish gut microbiota analysis differentiates physiology and behavior of invasive Asian carp and indigenous American fish. ISME J., 8, 541–551.
Martínez-Palacios, C.A., Ríos-Durán Ma, G., Campos-Mendoza, A., Toledo-Cuevas, M., Ross, L.G., 2002. Avances en el cultivo del pescado blanco de Pátzcuaro Chirostoma estor estor. In: Cruz-Suárez, L.E., Ricque-Marie, D., Tapia-Salazar, M., Gaxiola-Cortés, M.G., Simoes, N. (Eds.). Avances en Nutrición Acuícola VI. Memorias del VI Simposium Internacional de Nutrición Acuícola. 3 to 6 September. Cancún, Quintana Roo, México.
McFarland, L., Beneda, H., Clarridge, J., Raugi, G., 2007. Implications of the changing face of Clostridium difficile disease for health care practitioners. Am. J. Infect. Control., 35, 237–253.
Monroy, M.C., Castro, T., Fernández, F., y Mayorga, L., 2010. Inhibition of Aeromonas hydrophila by probiotic strains isolated from the digestive tract of Pterophyllumscalare. In Revista Mexicana de Ingeniería Química. 9(1), 37-42.
Mukherjee, A., Ghosh, K., 2014. Antagonism against fish pathogens by cellular components and verification of probiotic properties in autochthonous bacteria isolated from the gut of an Indian major carp, Catlacatla (Hamilton). Aquaculture. Res., 1-13.
Munro, L., 1982. The pathogenesis of bacterial disease of fishes. En Ronald, J. (comp.), Microbial diseases of fishes. Academic Press, London. 80.
Nayak, S.K., 2010. Role of gastrointestinal microbiota in fish. Aquaculture. Res., 41(11), 1553–1573.
Paniagua, G.L., Monroy, E., Perches, M., Negrete, E., García, O., Vaca, S., 2006. Antibiotic and heavy metal resistance of Aeromonas hydrophila isolated from charal (Chirostomahumboldtianum, Valenciannes, 1835), Hidrobiológica, 16(1), 75-79.
Perdikaris, C., Nathanailides, C., Gouva, E., Ujagwung, G., Bitchava, K., Athanasopoulou, F., Paschou, A., y Paschos, L., 2010. Size-relative effectiveness of clove oil as an anaesthetic for rainbow trout (Oncorhynchus mykiss Walbaum, 1792) and Goldfish (Carassius auratus Linnaeus, 1758). Acta. Vet., 79, 481–490.
Prasanna, R., Madhan, K., Singh, R.N., Chauhan, A.K., Nain, L., 2010. Developing biochemical and molecular markers for cyanobacterial inoculants. Folia. Microbiol., 55, 474–480.
Ringø, E., Myklebustd, R., Mayhewe, T.M., Olsen, R.E., 2007. Bacterial translocation and pathogenesis in the digestive tract of larvae and fry. Aquaculture., 268, 251–264.
Sim, I., Koh, J., Kim, D., Gu, S., Park, A., y Lim, Y., 2015. In vitro assessment of the gastrointestinal tolerance and immunomodulatory function of Bacillus methylotrophicus isolated from a traditional Korean fermented soybean food. J. Appl. Microbiol., 118(3), 718-26.
Sugita, H., Okano, R., Suzuki, Y., Iwai, D., Mizukami, M., Akiyama, N., Matsuura, S., 2002. Antibacterial abilities of intestinal bacteria from larval and juvenile Japanese flounder against fish pathogens. Fish. Sci., 68, 1004-1011.
Sugita, H., Tsunohara, M., Ohkoshi, T., y Deguchi, Y., 1988. Establishment of an intestinal Microflora in developing goldfish (Carassius auratus) of culture ponds. Microbiol. Ecol., 15, 333-344.
Sun, Y.Z., Yang, H.L., Ma, R.L., Lin, W.Y., 2010. Probiotic applications of two dominant gut Bacillus strains with antagonistic activity improved the growth performance and immune responses of grouper Epinepheluscoioides. Fish. Shellfish. Immunol., 29, 803-809.
Verschuere, L., Heang, H., Criel, G., Dafnis, S., Sorgeloos, P., Verstraete, W., 2000. Protection of Artemia against the pathogenic effects of Vibrio proteolyticus CW8T2 by selected bacterial strains. Appl. Environ. Microbiol., 66, 1139-1146.
Wu, S., Yuan, L., Zhang, Y., Liu, F., Li, G., Wen, K., Kocher, J., Yang, X., y Sun, J., 2013. Gut Pathogens. 5, 22.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Monroy Dosta María del Carmen, Javier Velasco Sarabia, Diego Alberto Retana Ortega, María de los Ángeles Peralta, Margarita Hernández Martínez, Gustavo Alejandro Rodríguez Montes de Oca, José Cristóbal Román Reyes
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
