Chlorpyrifos-induced testicular damage in rats: The effect of glutathione antioxidant


  • E. E. Elsharkawy Departement of Fronsic Med. and Toxicology fac. of Vet. Med. Assuit University., Egypt
  • D. Yahia Departement of Fronsic Med. and Toxicology fac. of Vet. Med. Assuit University., Egypt
  • N.A. El-Nisr Animal Health Institute of Research- Egypt


Chlorpyriphos, Testosterone, Alkaline phosphatase, Oxidative stress, Spermatogenesis


This study investigated the induction of oxidative stress in the testis of adult rat exposed to Chlorpyriphos (CPF). CPF was administered orally, in a dose of 30 mg/kg body weight to male rats for 90 days/ twice/ weekly. Co- administration of water soluble non enzymatic antioxidant glutathione (GSH) was given in a dose of 100 mg/kg body weight, oral, for the same period. Another two groups of male rats were administered GSH and corn oil, respectively. The activities of superoxide dismutase and glutathione reductase were decreased while the levels of lipid peroxidation were increased in the testicular tissues of the exposed animals. Testosterone hormone level in the serum was significantly decreased. The decrease in the histochemical determination of testicular alkaline phosphatase was observed in CPF-treated rats. A significant decrease in all stages of spermatogenesis in the seminiferous tubules was recorded in the exposed animals. Co-dministration of GSH restored these parameters.


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How to Cite

E. Elsharkawy, E. ., Yahia, D. ., & El-Nisr, N. . (2012). Chlorpyrifos-induced testicular damage in rats: The effect of glutathione antioxidant. Scientific Journal of Veterinary Advances, 1(1), 1-11. Retrieved from



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