Evaluation the bovine oviduct epithelial cells culture in monolayer system
Keywords:monolayer, oviduct, viability, proliferation
The oviduct is an important site in reproduction, such as fertilization and early embryonic development. In this study bovine oviduct epithelial cells (BOEC) isolated and cultured in monolayer system to evaluate the proliferation and viability of cells in quality manner before and after two time passages. The epithelial cells isolated mechanically from oviduct tissue in sterile condition. Isolated cells floated in culture medium, fetal bovine serum and antibiotics in a T-24 culture flask. Two days after seeding have been observed that cells adhered to flask surface and are proliferating. The rate of cell proliferation was well. After one week the cells covered the flask surface and were confluent. Then the cells isolated from flask by trypsin-EDTA enzyme and passage into 4 flasks. Cells cover the flasks in 10 days and passage into 12 flasks. In the second passage the cells was confluent in 12 days. We observed that in first culture there was a little dead cell during the culture and cells proliferation rate was high. After each passage the number of dead cells in flasks increased and cells proliferation rate in each passage decreased.
Ellington, J.E., 1991. The bovine oviduct and its role in reproduction - a review of the literature. Cornell Veter., 81,
Hiroyuki, A., Hiroyoshi, H., 1997. Bovine oviductal epithelial cells: their cell culture and applications in studies for
reproductive biology. J. Cytotech., 23, 171–183.
Hishinuma, M., Takahashi, Y., Kanagawa, H., 1989. Isolation and monolayer culture of bovine oviduct epithelial
cells. Jpn. J. Vet. Sci., 51, 1201–1208.
Schoen, J., Bondzio, A., Topp, K., Einspanier. R., 2008. Establishment and characterization of an adherent pure
epithelial cell line derived from the bovine oviduct. J. Theriogenol., 69, 536–545. Joshi, M. S., 1988. Isolation,
cell culture and immunocytochemical characterization of oviduct epithelial cells of the cow. J. Reprod. Fertil.
Myers, M.W., Broussard, J.R., Menezo, Y., Prough, S.G., Blackwell, J., Godke, R.A., Thibodeaux, J.K., 1994.
Established cell lines and their conditioned media support bovine embryo development during in-vitro
culture. J. Hum. Reprod., 9, 1927–1931.
Patrone, L.M., Cook, J.R., Crute, B.E., Van Buskirk, R.G., 1992. Differentiation of epithelial cells on microporous
membranes. J. Tissue. Cult. Methods., 14, 225–234.
Reischl, J., Prelle, K., Schöl, H., Neumüller, C., Einspanier, R., Sinowatz, F., Wolf, E., 1999. Factors affecting
proliferation and dedifferentiation of primary bovine oviduct epithelial cells in vitro. J. Cell. Tissue. Res., 296,
Villars, F., Conrad, V., Rouais, F., Lefebvre, F., Amédée, J., Bordenave, L., 1996. Ability of various inserts to promote
endothelium cell culture for the establishment of co-culture models. Cell. Biol. Toxicol., 12, 207–214.
Walter, I., 1995. Culture of bovine oviduct epithelial cells (BOEC). J. Anat. Rec., 243, 347–356
Xu, K.P., Yadav, B.R., Rorie, R.W., Plante, L., Betteridge, K.J., King, W.A., 1992. Development and viability of bovine
embryos derived from oocytes matured and fertilized in vitro and co-cultured with bovine oviductal epithelial
cells. J. Reprod. Fertil., 94, 33–43.
How to Cite
Copyright (c) 2014 Shiva Erfani, M. T. Harkinezhad, Hamid Amanlou
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.