Effects of methanolic leaf extract of Telfairia occidentalis (Hook f.) on the reproductive indices of female albino rats
Keywords:reproductive indices, gestation, repro-toxicity, progesterone
This study investigated the effects of oral administration of methanolic leaf extract of Telfairia occidentalis (MLETO) on the reproductive indices of female albino rats. Fifty-six Sprague-Dawley albino rats (42 females and 14 males) were used for the study. The females were randomly assigned into 7 groups (designated A, B, C, D, E, F and G). Group A received only distilled water, while groups B and C received 200 and 800 mg/kg body weight of MLETO for the first 7 days of gestation; groups D and E received 200 and 800 mg/kg of MLETO for the first 14 days, and groups F and G received 200 and 800 mg/kg of MLETO for the entire 21 days of gestation respectively. The males were only used in mating the females. The number of return to estrus per pregnancy, number of implanted/developing conceptuses, gestation length, litter size at birth, litter weight at birth, crown-rump length at birth and aborting rat were assessed. Quantal pregnancy, implantation index, viability index, birth index, live-birth index and percentage aborted were calculated. Serum progesterone was also assayed. There were no significant (P > 0.05) variations between the groups in all the reproductive indices assessed and also on the serum progesterone level. It was concluded that administration of MLETO as used in this study had no significant effect on the reproductive indices and serum progesterone levels of pregnant rats.
Aronson, J. K. 2009. Meyler’s Side Effects of Herbal Medicines. Elsevier. Netherlands.
Canadian Council on Animal Care, 2003. Guideline on laboratory animal facilities-characteristics, design and development. http://www.ccac.ca.
Dog, L. T. 2009. The use of botanicals during pregnancy and lactation. Altern Ther Health Med. 15 (1): 54 – 58.
Dogoua, J. J. 2010. Herbal medicines and pregnancy. J Popul Ther Clin Pharmacol. Fall; 17(3): 370 – 3788.
Ehiagbonare, J. E. 2008. Conservation studies on Telfairia occidentalis Hook f. an indigenous plant used in ethnomedical treatment of anemia in Nigeria. African Journal of Agricultural Research 3(1): 74-77.
Food and Agriculture Organisation of the United Nations (FAO). 2007. Helping to build a world without hunger.TAO. Telfairia occidentalis data sheet.
Kayode, A. A., Kayode, O. T. 2010. Some Medicinal Values of Telfaria occidentalis: A review. American Journal of Biochemistry and Molecular Biology. 1: 30-38.
Lorke, D. 1983. A New Approach to Practical Acute Toxicity Testing. Archives of Toxicology. 54: 275 – 287.
Magness, R. R. 1998. Maternal cardiovascular and other physiologic responses to the endocrinology of pregnancy. in: Bazer, F. W (Ed), Endocrinology of pregnancy. Humana Press Inc. New Jersey. Pp. 507-540.
Marcus D. M., Snodgrass, W. R. 2005. Do no harm: avoidance of herbal medicines during pregnancy. Obstet Gynecol. 105(5): 1119 – 1122.
Mendes, C. C., Marinho, C. M., Moreira-Junior, V. F., Danta G. L., Macedo M. F., Oliveira C. N., Schwarz, A. 2010. Evaluation of Bauhinia monandra aqueous and ethanol extracts in pregnant rats. Pharm Biol. Doi: 10.3109/13880200903280018
Mensah, J. K, Okoli, R I., Ohaju-Obodo K. E. 2008. Phytochemical, nutritional and medical properties of some leafy vegetable consumed by Edo people of Nigeria. African Journal of Biotechnology. 7(14): 2304-2309.
Monobind Inc. 2011. Instruments and Application Instruction leaflet. www.monobind.com.
National Health and Medical Research Council, (NHMRC). 2008. Guidelines to promote the wellbeing of animals used for scientific purposes: the assessment and alleviation of pain and distress in research animals. Australian Government.
Oboh, G., Nwanna, E. E., Elusiyan, C. A. 2006. Antioxidant and Antimicrobial Properties of Telfairia occidentalis (Fluted pumpkin) Leaf Extracts. Journal of Pharmacology and Toxicology 1(2): 167-175.
Ochiogu, I. S., Uchendu, C. N., Ihedioha, J. I. 2006. A new and simple method of Confirmatory detection of mating in albino rats (Rattus norvegicus). Animal Research International. 3(3): 527 -530.
Ogunlesi, M., Okiei, W., Azeez, L., Obakachi, V., Osunsanmi, O., Nkechor, G. 2010. Vitamin C content of tropical vegetable and food determined by voltametric and titrimetric methods and their relevance to the medicinal uses of the plant. Int. J. Electrochem. Sci. 5: 105-115.
Olaniyan, M. F., Adeleke, A. 2005. A Study of the effect of pumpkin (ugu- Telfaria occidentalis) milk and raw egg mixture in the treatment of anaemic pregnant women in a rural area. African Journal of Traditional, Complementary and Alternative Medicines. 2(3): 269-273.
Olaniyi, T. O., Odedere, M. P. 2009. The effect of mineral Nitrogen and compost fertilizers on the growth, yield and nutritional values of fluted pumpkin (Telfaria occidentalis) in South Western Nigeria. Journal of Animal and Plant Sciences. 5(1): 443-449.
Omale, J., Okafor, P. 2009. Cytotoxicity and antioxidant screening of some selected Nigerian medicinal plants. Asian Journal of Pharmaceutical and Clinical Research. 2(4): 48 – 53.
Oyeyemi, M., Leigh, O., Ajala, O., Badejo, A., Emikpe, B. 2008. The effects of the aqueous extract of ‘ugu’ (Telfairia occidentalis) leaves on the testis and spermatozoa characteristics in the male albino rat. Folia Veterinaria. 52 (2): 102-105.
Pineda, M. H. 1989. Male Reproduction, in: McDonald, L. E., Pineda, M. H. (Eds), Mcdonald’s Veterinary Endocrinology and Reproduction, fourth ed. Lea and Febiger, London. Pp. 264.
Ratnasooriya, W., Jayakody, J., Premakumara, G. 2003. Adverse pregnancy outcome in rats following exposure to a Salacia reticulate (Celastaceae) root extract. Brazilian Journal of Medical and Biological Research. 36: 931-935.
Saalu, L. C., Kpela, T., Benebo, A., Oyewopo, A., Anifowpe, E., Oguntola, J. 2010. The dose-dependent testiculo-protective and testiculo-toxic potentials of Telfairia occidentalis Hook f. leaves extract in rats. International Journal of Applied Research in Natural Products.3(3): 27-28.
Salman, T. M., Olayiki, A. L., and Oyeyemi, W. A. 2008. Aqueous extract of Telfairia occidentalis leaves reduces blood sugar and increases haematological and reproductive indices in male rats. African Journal of Biotechnology. 7(14): 2299-2303.
Tindall, H. 1986. Commercial Vegetable Growing. Oxford Press. London. Pp. 69.
Ukwuoma, B., Muanya C. 2005. Fighting degenerative diseases with vegetable soup. www.guardiannewsngr.com/naturalhealth.article01/13075.
How to Cite
Copyright (c) 2015 C. N. Okoye, D. Ogwu, J. I. Ihedioha, I. S. Ochiogu, C. N. Abiaezute, E. C. Mbegbu
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.