In-vitro antibacterial evaluation of ethanolic stem crude extracts of anacardium occidentale linn. (anacardiaceae) on streptococcus mutans associated with dental caries
Keywords:
Streptococcus mutans, Cashew, Caries, Phytochemical, AntibacterialAbstract
The preliminary phytochemistry and antibacterial activity of ethanolic stem crude extracts of Anacardium occidentale on Streptococcus mutans isolated from dental caries were determined using chemical, standard microbiological and agar disc diffusion techniques. The phytochemical analysis of the ethanolic stem crude extracts of Anacardium occidentale revealed the presence of alkaloids (+++), phenolics (+++), saponins (+), tannins (++), flavonoids (++), phlobatanins (+), anthraquinones (+), terpenes (++), deoxy-sugar (++) and cardiac glycosides (+). The results showed that between 65.2% to 95.6% of S. mutans isolated were sensitive to different concentrations of ethanolic stem crude extracts of A. occidentale with Activity Index (A.I) ranging from 0.38 to 1.22. Ethanolic stem crude extracts of A. occidentale showed the highest mean zones of Inhibition (17.9 ± 1.3mm) at 50.0 mgml-1 and lowest inhibition diameter (6.5±1.5mm) at 12.5 mgml-1 on S. mutans, thus, exhibiting concentration dependent activity. The results show that S. mutans with code WD09 was resistant to Streptomycin, Amoxycillin, di-methyl sulphoxide and A. occidentale ethanolic stem crude extracts. Therefore, there is a need to consider the use of this potent ethanolic stem crude extracts of Anacardium occidentale that have shown some measures of antimicrobial potency, judging by the antibacterial activity and activity index for developing synthetic drugs against dental caries caused by Streptococcus mutans.References
Adeleye, I.A., Ogunwale, O.O., 2004. Isolation and antibiotic susceptibility of aerobic bacterial pathogen associated
with wound infections in Lagos. Biosci. Res. Commun. 16, 1-5.
Ahmad, I., Beg, Z.A., 2001. Antimicrobial and phytochemical studies of 45-Indian medicinal plants against multidrug resistant human pathogens. J. Ethnopharm. 74, 113-123.
Akinjogunla, O.J., Adegoke, A.A., Udokang, I.P., Adebayo-Tayo, B.C., 2009. Antimicrobial potential of Nymphaea
lotus (Nymphaeaceae) against wound pathogens. J. Med. Plant. Res. 3(3), 138-141.
Akinjogunla, O.J., Ekoi, O.H., Odeyemi, A.T., 2011. Phytochemical screening and in-vitro antibacterial assessment of
aqueous leaf extracts of Vernonia amygdalina (Asteraceae) and Ocimum gratissimum (Lamiaceae) on
moxifloxacin resistant E. coli isolated from clinical and environmental samples. Natu. Sci. 9(7), 42-52.
Arekemase, M.O., 2011. Antibacterial Activity of Anacardium occidentale on some enterotoxin producing bacteria.
Inter. J. Biol. 3(4), 92-99
Akinpelu, D.A., 2001. Antimicrobial activity of Anarcardium occidentale bark. In Fitoterapia, 72 (3),286-287.
Ali, M.A., Alam, N.M., Yeasmin, S., Khan, A.M., Sayeed, M.A., 2007. Antimicrobial screening of different extracts of
Piper longum Linn. Res. J. Agri. Biol. Sci. 3 (60), 852-857.
Atata, R.F., Sani, H., 2003. Effects of stem bark extracts of Enantia chloranta on some clinical isolates. Biokemistri,
, 84-92.
Bilcalho, B., 2001. Volatile compounds of cashew apple (Anarcardium occidentale L.). Z. Naturforsch, 56(1-2), 35-
Chakraborthy, G.S., 2008. Antimicrobial activity of the leaf extracts of Calendula officinalis (Linn.), J. Herb. Med.
Toxicol. 2(2), 65-66.
Cheesbrough, M., 2006. District Laboratory practice in tropical countries (Part II). Cambridge University pp 19-110
Cowan, S., 1999. Cowan and Steels Manual for the Identification of Medical Bacteria. (5th Edn) Cambridge
University Press, Cambridge, London, p. 89-112.
Cragg, G.M., Boyd, M.R., Khanna, R., Kneller, R., Mays, T.D., Mazan, K.D., Newman, D.J., Sausville, E.A., 1999.
International collaboration in drug discovery and development: the NCI experience, Pure and Applied
Chemistry, 71, 1619-1633.
Cushnie, T.P., Lamb, A.J., 2005. Antimicrobial activities of flavonoids. Inter. J. Antimicrob. Agent. 26, 343.
Fawole, M.O.,Oso, B.A., 1988. Laboratory Manual for Microbiology (1st Edition), Spectrum Book Ltd, Ibadan, p. 22-
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Stately, J.T., Williams, S.T., 1994. Bergey’s Manual of Determinative
Bacteriology, (9th edn), Baltimore, Williams and Wilkins. p.787.
Jagtap, S.D., Deokule, S.S., Pawar, P.K. and Harsulkar, A.M., 2009. Traditional Ethnomedicinal Knowledge Confined
to the Pawra Tribe of Satpura Hills, Maharashtra, India. Ethnobot. Leaflet., 13, 98-115.
Junaid, S.A., Olabode, A.O., Onwuliri, F. C., Okwori, A.E.J., Agina, S.E., 2006. The antimicrobial properties
of Ocimum gratissimum gastrointestinal isolates. In African J. Biotechnol. 5(22), 2315-2321.
Kornman, K.S., 1986. The role of supragengival plaque in the prevention and treatment of periodontal diseases: a
review of current concepts. J. Periodon. Res. Supl, p. 5-22.
Marsh, P.P., 1992. Microbiological aspects of the chemical control of plaque and gingivitis. J. Dental Res. 71, 1431-
Morton, J.F., 1987. Fruits of Warm Climates. Flair Books, Florida, ISBN: 0-9610184-1-0.
NCCLS – National Committee for Clinical Laboratory Standards (2004). Performance standards for Antimicrobial
susceptibility testing. Fourteenth informational supplemented. M100-S14, Wayne, PA, USA.
Newman, D.J., Cragg, G.M., Sander, K.M., 2000. The influence of natural products upon drug discovery. Natur.
Prod. Res. 17, 215-234.
Okada, M., Soda, Y., Hayashi, F., Doi, T., Suzuki, J., Miura, K. and Kozai, K., 2005. Longitudinal study of dental
incidence associated with Streptococcus mutans and Streptococcus sobrinus in pre-school children. J. Med.
Microb. 54, 661-665.
Okigbo, R.N., Ogbonnanya, O.U., 2006. Antifungal effects of two tropical plants extracts Ocimum gratissimum and
Afromaomum melegueta on post harvest yam Discorea spp rot. African J. Biotechnol. 5(9), 727-731.
Olowosulu, A.K., Ibrahim, Y.K.E., 2006. Studies on the antimicrobial screening of aqueous extracts of five plants
used in folk medicine in Nigeria. West African J. Biol. Sci. 3(5), 21-26.
Omojasola, P.F., AWE, S., 2004. The antibacterial activity of the leaf extracts of Anacardium occidentale and
Gossypium hirsutun against some selected microorganisms. Biosci. Res. Commun. 60 (1), 25-58.
Opeke, L.K., 2005. Tropical commodity. Tree Crops. 107 – 109
Ophori, E.A., Eriagbonye, B.N., Ugbodaga, P., 2010. Antimicrobial activity of propolis against Streptococcus mutans.
African J. Biotechnol. 9(31), 4966-4969.
Petersen, P.E., Bourgeois, D., Ogawa, H., Estupinan-Day, S., Ndiaye, C., 2005. The global burden of oral diseases
and risks to oral health. Bull. World Heal. Organiz. 83, 661-669.
Piddock, K.J.V., Wise, R., 1989. Mechanism of resistance to quinolones and clinical perspective. J. Antimicrob.
Chemoth. 23, 475-483.
Raskin, L., Ribnicky, D.M., Komarnytsky, S., Llin, N., Poulev, A., Borisjuk, N., Brinker, A., Moreno, D.A., Ripoll, C.,
Yakoby, N., O’Neal, J.M., Cornwell, T., Pastor, I., Fridlender, B., 2002. Plants and human health in the twenty
first century. Trend. Biotechnol. 20 (12), 522-531.
Service, R.F., 1995. Antibiotics that resist resistance. Sci. 270, 824-727.
Sofowora, A., 1994. Medicinal plants and traditional medicines in Africa. Chichester John, Wiley and Sons New
York, p. 256.
Trease, G.E., Evans, W.C., 1989. Pharmacology (11th Ed). Bailliere Tindall Ltd, London. pp 60-75. In: Westh, H., Zinn,
C.S., Rosahi, V.T. An international multicenter study of antimicrobial consumption and resistance in
Staphylococcus aureus isolates from 15 hospitals in 14 countries. Microb. Drug Res. 10, 169 –179.
Yusuf, S., Aliyu, M., Ndanosa, R., 2009. Effect of aqueous extract of Anarcardium occidentale (L) stem bark on
sodium and chloride transport in the rabbit colon. J. Med. Plant. Res. 3(6),493-497.
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