Characterization of Escherichia coli isolates obtained from washing and rinsed water of broilers in pluck shops at Sreepur of Gazipur district in Bangladesh
Keywords:
Characterization, Escherichia coli, Washing and rinsed water of broilers, Pluck shopsAbstract
The study was aimed at characterization of Escherichia coli isolates obtained from washing and rinsed water of broilers in pluck shops at Sreepur of Gazipur district in Bangladesh. A total of 30 samples collected from the different layers of drums of pluck shops’ were subjected to bacterial isolation and identification by using cultural and biochemical techniques. Furthermore, the isolated E. coli strains were characterized by antimicrobial susceptibility testing. The fermentation reaction by the isolates of E. coli in five basic sugars (dextrose, sucrose, fructose, maltose, mannitol) and in dulcitol were positive. Moreover, methyl red reaction and catalase tests were also positive for E. coli. On the other hand, E. coli was prevailed in upper layer of drums (31.82%), in middle layer of drums (36.36%) and in lower layer of drums (31.82%). E. coli isolates were resistant to erythromycin and enrofloxacin. However, most of the E. coli isolates were susceptible to sulfamexazole-trimethoprim and gentamycin. Out of 22 E. coli isolates, 16 (72.73%) were multidrug resistant. The findings of the study revealed the presence of multidrug resistant E. coli isolates in washing and rinsed water of broilers in Pluck shops at Sreepur of Gazipur district in Bangladesh.
References
Ali, M.Y., Rahman, M.T., Islam, M.A., Choudhury, K.A., Rahman, M.A., 1998. Characteristics of E. coli isolates of
human and animal origin. Progressive Agri. 9, 221-224.
Bangladesh Bureau of Statistics, 1996. Statistical Year Book of Bangladesh. Seventeenth Edition.
Bauer, A.W., Kirby, W.M., Sherris, J.C., 1966. Antibiotic susceptibility testing by a standard single disc method.
American Journal of Clinical Pathology, 45, 493-496.
Bensink, J.C., Botham, F.P., 1983. Antibiotic resistant coliform bacilli, isolated from freshly slaughtered poultry and
from chilled poultry at retail outlets. Aust Vet. J. 60, 80-83.
Beutin, L., Geier, D., Zimmeronann, S., Aleksic, S., Gillespie, H.A, Whittam, T.S., 1997. Epidemiological relatedness
and clonal types of natural populations of E. coli strains producing shiga toxins in separate population of
cattle of sheep. Appl. Environ. Microbiol. 63, 2175-2180.
Caudry, S.D., Stanisich, V.A., 1979. Incidence of antibiotic resistant Escherichia coli associated with frozen chicken
carcasses and characterization of conjugative R-plasmids derived from such strains. Antimicrob. Agent.
Chemother. 16, 701-709.
Cheesbrough, M., 2000. District laboratory practice in tropical countries (part 2). Cambridge University Press, UK,
pp. 62-70.
Cowan, S.T., 1985. Cowan and Steels manual for identification of bacteria (2nd ed). Cambrige University press, UK.
Escherich, T., 1885. "Die Darmbakterien des Neugeborenen und Säuglinge". Fortschr Med. 3, 515–522.
Islam, M.T., Islam, M.A., Samad, M.A., Kabir, S.M.L., 2004. Characterization and antibiogram of Escherichia coli
associated with mortality in broilers and ducklings in Bangladesh. Bang. J. Vet. Med. 2, 09-14.
Kabir, S.M.L. 2010. Avian colibacillosis and salmonellosis: a closer look at epidemiology, pathogenesis, diagnosis,
control and public health concerns. Int. J. Environ. Res. Public. Health. 7, 89-114.
Linton, A.H., Howe, K., Hartley, C.L., Clements, H.M., Richmond, M.H., Osborne, A.D., 1977. Antibiotic resistance
among Escherichia coli O-serotypes from the gut and carcasses of commercially slaughtered broiler chickens:
a potential public health hazard. J. Appl. Bacteriol. 42, 365-378.
Hossain, M.T., 2006. Characterization, toxin profile and antibiogram of Escherichia Coli isolated from chicken. M.S.
Thesis, Department of Microbiology and Hygiene, Faculty of Veterinary Sciences, Bangladesh Agricultural
University, Mymensingh.
Nazer, A.H., 1980. Transmissible drug resistance in Escherichia coli isolated from poultry and their carcasses in Iran.
Cornell Vet., 70, 365-371.
Nazir, K.H.M.N.H., Rahman, M.B., Nasiruddin, K.M., Akhtar, F., Khan, M.F.R., Islam, M.S., 2005. Antibiotic sensitivity
of Escherichia coli isolated from water and its relation with plasmid profile analysis. Pak. J. Biol. Sci. 8, 1610-
NCCLS, 2000. Performance Standards for Antimicrobial Disk Susceptibility tests. Approved Standard - Seventh Ed.
, 1.
Quinn, P.J., Carter, M.E., Markey, B.K., Carter, G.R., 2000. Clinical veterinary microbiology. London, Mosby-year
book Europe limited, pp. 120-121.
Rahman, M.A., Samad, M.A., Rahman, M.B., Kabir, S.M.L., 2004. In vitro antibiotic sensitivity and therapeutic
efficacy of experimental salmonellosis, colibacillosis and pasteurellosis in broiler chickens. Bangl. J. Vet. Med.
, 99-102.
Sandhu, K.S., Clarke, R.C. McFadden, K., Brouwer, A., Louie, M.,Wilson, J., Lior, H, Gyles, C.L., 1996. Prevalence of
the eaaA gene in verotoxigenic E. coli strain in dairy cattle in South-West Ontario. Epidemio and Infect., 116,
-7.
Tollefson, L., Altekruse, S.F., Potter, M.E., 1997. Therapeutic antibiotics in animal feeds and antibiotic resistance.
Revue Scientifique et Technique, 16, 709-715.
Turtura, G.C., Massa, S., Chazvinizadeh, H., 1990. Antibiotic resistance among coliform bacteria isolated from
carcasses of commercially slaughtered chickens. Int. J. Food Microbiol. 11, 351-354.
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