Antimicrobial activity bioactive compounds produced by Exiguobacterium acetylicum PTCC1756 against pathogenic bacteria

Abstract

Almost all forms of life in the marine environment (bacteria, algae, fungi, etc…) have been investigated for their natural product content. In the last several decades, plants, animals and microbes from the marine environment have revealed a portion of what is clearly a tremendous source of structurally diverse and bioactive secondary metabolites. Exiguobacterium acetylicum is a Gram positive, rhizospheric, yellow pigmented bacteria isolated from an apple orchard rhizospheric soil, facultative aerobic, motile with peritrichous flagella and their growth ranges are from _2.5 to 40 . These micro organisms In separate invitro assays it was found to inhibit the growth and development of Rhizoctonia solani, Sclerotium rolfsii, Pythium and Fusarium oxysporum. The volatile compound produced by the bacterium was found to be the most potent in inhibiting the hyphal fungus and pathogens bacteria. Present study aims to isolated and detect the surfactants produced by Exiguobacterium acetylicum PTCC 1756 For this purpose, a suspension equal to 0.5 McFarland was prepared from the fresh growth of following standard clinic strains, Escherichia coli PTCC 1533, Shigella Flexneri PTCC 1234, Staphylococcus aureous PTCC 1112,  Salmonella enterica subsp. enterica serovar Paratyphi B PTCC 1231 and the plated on the Muller Hinton agar.  Supernatant of E. acetylicum growth was prepared in 15000 ppm for 30 minutes and was utilized. To study on the antimicrobial activity of  E. acetylicum PTCC1756, Muller- Hinton agar was used. For this purpose, Disk Diffusion Agar and Well Diffusion Agar techniques were used to define MIC. These micro organisms growth supernatant displayed a good antimicrobial property and the maximum effect was observed during the well diffusion technique against the standard strain Salmonella enterica subsp enterica serovar paratyphi PTCC 1231 with 8.5 mm no growth halo.