Polymorphism of Flagellin gene (fliC) of Clostridium chauvoei isolates from Iran
Keywords:
Clostridium chauvoei, Flagellin, PolymorphismAbstract
Flagellums have been reported to play a critical role in the protective immunity in host. In clostridia genera it is coded by flagellin (fliC) gene. Previous southern blot analysis has suggested that Flic gene may has two copies in genome of C. chauvoei. We studied sequences of two copies (fliA and fliB) of flagellin gene in Iranian isolates. In this study, four specific primers for fliA, fliB copies and two universal primers for flagellin (fliC) were designed. Polymerase chain reactions were performed to amplification of fliA and fliB. Two copies of flagellin gene were sequenced and compared with other sequences in GenBank. In phylogenic tree analysis, fliA and fliB sequences were located in separated (clusters) branches. Nucleotide sequence alignment showed that more divergence in fliBs than fliAs sequences. Six highly conserve regions and thirty-one single nucleotide polymorphisms (include thirty specific nucleotide pattern) were found between fliA and fliB sequences. These findings are the first report of Thirty-one novel Single Nucleotide Polymorphisms in fliC gene of Clostridium chauvoei isolates from Iran.
References
Alm, R.A., Guerry, P., 1993. Distribution and polymorphism of the flagellin genes from isolates of Campylobacter coli and Campylobacter jejuni. J. bacteriol., 175, 3051-3057.
Attridge, S., Rowley, D., 1983. The role of the flagellum in the adherence of Vibrio cholera. J. Infect. Dis., 147, 864.
Bagge, E., Lewerin, S.S., Johansson, K., 2009, 'Detection and identification by PCR of Clostridium chauvoei in clinical isolates, bovine faeces and substrates from biogas plant. Acta. Vet. Scand., 51, 8.
Joys, T.M., 1988. The flagellar filament protein. Can. J. Microbiol., 34, 452-458.
Kojima, A., Uchida, I., Sekizaki, T., Sasaki, Y., Ogikubo, Y., Kijima, M., 2000. Cloning and expression of a gene encoding the flagellin of Clostridium chauvoei. Vet. Microbiol., 76, 359-372.
Kojima, A., Uchida, I., Sekizaki, T., Sasaki, Y., Ogikubo, Y., Tamura, Y., 2001. Rapid detection and identification of Clostridium chauvoei by PCR based on flagellin gene sequence. Vet. Microbiol., 78, 363-371.
Kostrzynska, M., Betts, J.D., Austin, J., Trust, T.J., 1991. Identification, characterization, and spatial localization of two flagellin species in Helicobacter pylori flagella. J. Bacter., 173, 937-946.
Macnab, R.M., 2004. Type III flagellar protein export and flagellar assembly' Biochimica et Biophysica Acta (BBA). Mol. Cell. Res., 1694, 207-217.
Miyashiro, S., Nassar, A., Souza, M., Carvalho, J., Adegas, J., 2007. Identification of Clostridium chauvoei in clinical samples cultures from blackleg cases by means of PCR. Brazil. J. Microbiol., 38. 491-493.
Morooka, T., Umeda, A., Amako, K., 1985. Motility as an intestinal colonization factor for Campylobacter jejuni. J. Gen. Microbiol., 131, 1973-1980.
Sasaki, Y., Kojima, A., Aoki, H., Ogikubo, Y., Takikawa, N., Tamura, Y., 2002. Phylogenetic analysis and PCR detection of Clostridium chauvoei, Clostridium haemolyticum, Clostridium novyi types A and B, and Clostridium septicum based on the flagellin gene. Vet. Microbiol., 86, 257-267.
Tamura, Y., Tanaka, S., 1984. Effect of antiflagellar serum in the protection of mice against Clostridium chauvoei. Infect. Immun., 43, 612-616.
Tanaka, M., Hirayama, N., Tamura, Y., 1987. Production, characterization, and protective effect of monoclonal antibodies to Clostridium chauvoei flagella. Infect. Immun., 55, 1779-1783.
Tino, T., 1977. Genetics of structure and function of bacterial flagella. Ann. Rev. Genet., 11, 161-182.
Wilson, G.S., Miles, A.A., 1975.Topley and Wilson's principles of bacteriology. Virol. Immune., p, 345
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Copyright (c) 2014 Ahmad- Reza Jabbari, Khalil Azizian, Majid Esmaelizad
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