Parasitemia and Antimalarial-Induced Histological Alterations and Oxidative stress in Infected Mice
Keywords:
Plasmodium berghei, antimalaria, oxidative stress, organs, miceAbstract
In lieu of the reports on re-emergence of chloroquine sensitivePlasmodium falciparum, the choice of the drug for the treatment of malariaespecially in the endemic areas may not be ruled out. Here, we reported the antioxidative statusand histology of some vital organs in mice infected with P. berghei and subsequentlytreated with chloroquine. The study was a 4 by 10 model design as follows, thecontrol non-infected, the infected (IN) and chloroquine-treated infected(CqTI)/non-infected (CqTNI) mice. The challenged animals were subjected to fivedays treatment after parasitemia was established using Gemsa stain. Assays wereconducted on the animals following standard procedures. The results showed thatparasitemia and Chloroquine induced oxidative stress in (IN) and (CqTNI)groups. This was indicated by significant (p<0.05) changes in theantioxidant defence indices viz superoxide dismutase (SOD), reduced glutathione(GSH), glutathione-S-transferase (GST), catalase (CAT), malondialdehyde (MDA)and protein concentration. There were also alterations in the architectures ofsome vital organs such as liver, kidney and heart of the experimental groupscompared with control. The subsequent treatment of the infected group withChloroquine (CqTI) restored some of the indices altered during the infection toa normal level. This study shows that malaria and its radical treatment in vivo can induce oxidative stress which inturn can cause injury to the host tissues particularly during chronicadministration of Chloroquine-an implication for prolong intake of the drug.
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Copyright (c) 2014 I.O. Oyewole, G.N. Anyasor, O.O. Aina, A.A. Ogunnowo, B.A. Sorinwa, B.E. Abraham
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