Histopathological study on the effects of oral administration of aqueous leaf extracts of cymbopogon citratus on the frontal cortex of male sprague dawley rats
Keywords:
Cymbopogon citratus, Cytoarchitecture, Leaf extract, Light microscopic, Oral consumption, Phosphate buffered salineAbstract
This investigation was to evaluate the histopathological effects of oral consumption of the aqueous leaf extract of Cymbopogon citratus on the frontal cortex of Sprague Dawley (SD) rats. Ten male SD rats weighing between 150-230g were used. The rats were randomly assigned into two groups designated as groups A and B. Group A served as the control group while group B was the treatment. 300 mg/kg body weight of the aqueous leaf extract of C. citratus was administered once daily for 14 consecutive days (14d) orally through a sterilized orogastric tube, while the control rats received equal volume of phosphate buffered saline (PBS). The rats were sacrificed 24 hours after the last administration. The frontal cortices were excised, fixed in 10% formol calcium for 18 hours and were processed for routine light microscopic study using Hematoxylin and Eosin (H&E) method. The histological findings revealed that the extract treated sections showed cytoarchitectural distortions ranging from neuronal degeneration, distortion, vacuolations and evidence of necrotic bodies while the sections from the rats in group B conform to normal histological profile. These findings suggest a deleterious and toxic effects of aqueous leaf extract of C. citratus on the frontal cortex of SD rats.
References
Adekomi, D.A., Tijani, A.A., Adeniyi, T.D., Olajide, J.O., 2011. Some of the Effects of Aqueous Leaf Extracts of
Cnidoscolus aconitifolius (Euphorbiaceae) on the Morphology and Histology of the Kidney and Liver of
Sprague Dawley Rat. Trop. J. Healt. Sci. 18, 9-15.
Blanco, M.M., Costa, C.A., Freire, A.O., Santos, J.G., Costa. M., 2009. Neurobehavioral Effect of Essential Oil of
Cymbopogon citratus in mice. Phytomedicine. 16 (2-3), 265–70.
Bleasel, N., Tate, B., Rademaker, M., 2002. Allergic contact dermatitis following exposure to essential oils.
Australas. J. Dermatol. 43 (3), 211–3.
Dapar, L.P.M., Aguiyi, C.J., Wannang, N.N., Gyang, S.S., Tanko, M.N., 2007. The Histopathologic Effects of
Securidaca longepedunculata on Heart, Liver, Kidney and Lungs of Rats. Afr. J. Biotechnol. 6 (5), 591-595
Dubey, N.K., Kishore, N., Varma, J., Lee, S.Y., 1997a. Cytotoxicity of the essential oils of Cymbopogon citratus and
Ocimum gratissimum. Indian J Pharm Sci. 59, 263-64.
Dubey, N.K., Tekeya, K., Itokawa, H., 1997b. Citral, A cytotoxic principle isolated from the essential oil of
Cymbopogon citratus against P388 leukemia cells. Curr Sci.73, 22-24.
Farber, J.L., Chein, K.R., Mittnacht, S., 1981. The pathogenesis of Irreversible cell injury in ischemia. Am. J. Pathol.
, 271-281.
Ito, U., Sparts, M., Walker, J.R., Warzo, I., 2003. Experimental cerebral ischemia in magolian gerbils (1), light
microscope observations. Acta Neuropathol. (USA). 32, 209-223.
Iwu, M.M., 2000. International conference on ethnomedicine and drug discovery. J Altern Complement Med. 6, 3-
Koffi, K., Komla, S., Catherine, G., Christine, R Jean-Pierre, C., Laurence, N., 2009. In vitro cytotoxic activity of
Cymbopogon citratus L. and Cymbopogon nardus L. Essential oils from Togo. Bangladesh J Pharmacol. 4, 29-
Leite, J.R., Seabra, M.L., Maluf, E., et al., 1986. Pharmacology of Lemongrass (Cymbopogon citratus Stapf). III.
Assessment of Eventual Toxic, Hypnotic and Anxiolytic Effects on Humans. J Ethnopharmacol. 17 (1), 75–83.
Lorenzetti, B.B., Souza, G.E.P., Sarti, S.J., Filho, D.S., Ferreira, S.H., 1991. Myrcene mimics the peripheral analgesic
activity of lemongrass tea. J. Ethnopharmacol. 34, 43-48.
Mishra, A.K., Dubey, N.K., 1994. Evaluation of some essential oils for their toxicity against fungi causing
deterioration of stored food commodities. Appl. Environ. Microbiol. 60, 1101-1105.
National Institutes of Health Guide for the Care and Use of Laboratory Animals: DHEW Publication (NIH), revised.
Office of Science and Health Reports, DRR/NIH, Bethesda, USA, 1985.
Negrelle, R.R.B., Gomes, E.C., 2007. Cymbopogon citratus (DC.) Stapf: Chemical Composition and Biological
Activities. Rev. Bras. Pl. Med. 9, 80-92.
Ofusori, D.A., Adelakun, A.E., Ayoka, A.O., Oluwayinka, O.P., Omotoso, E.O., Odukoya, S.A., Adeyemi, D.O., 2008.
Waterleaf (Talinum triangulare) Enhances Cerebral Functions in Swiss albino mice. Journal of Neurological
Sciences [Turkish]. 25; 239-246.
Onawunmi, G.O., Yisak, W.A., Ogunlana, E.O., 1984. Antibacterial constituents in the essential oil of Cymbopogon
citratus (DC.) Stapf. J. Ethnopharmacol. 12, 279-286.
Orafidiya, L.O,, Oyedele, A.O., Shittu, A.O., Elujoba, A.A., 2001. The Formulation of an Effective Topical
Antibacterial Product Containing Ocimum gratissimum Leaf Essential Oil. Int J Pharm. 224, 177-83.
Pereira, R.P., Fachinetto, R., de Souza, P.A., Puntel, R.L., Santos da Silva, G.N., Heinzmann, B.M., Boschetti, T.K.,
Athayde, M.L., Bürger, M.E., Morel, A.F., Morsch, V.M., Rocha, J.B., 2009. Antioxidant Effects of Different
Extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citratus. Neurochem Res. 34 (5), 973-
Shadab, Q., Hanif, M., Chaudhary, F.M., 1992. Antifungal activity by lemongrass essential oils. Pak. J. Sci. Ind. Res.
, 246-249.
Waters, C.M., 1994. Glutamate induced apoptosis of striatal cells in rodent model for Parkinsonism. Neuroscience.
, 1-5.
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