In vitro evaluation of the acaricidal effect of vegetal oils extracted from the kernel of Thevetia peruviana and Annona muricata on the Rhipicephalus (Boophilus) microplus larvae

Authors

  • Kossi Justin Adinci University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Yao Akpo University of Parakou (UP), Faculty of Agronomy, Po Box 123 Parakou, Benin
  • Arétas Tonouhewa University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Roland Eric Yessinou University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Philippe Sessou University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Mahuto Yovo Laboratory of Research and Study in Applied Chemistry. Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Benin
  • Safiou Bienvenu Adehan National Institute for Scientific Research, Research Center of Agonkanmey (CRA / INRAB), Abomey-Calavi, Benin
  • Camus Adoligbe University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Guy Appolinaire Mensah National Institute for Scientific Research, Research Center of Agonkanmey (CRA / INRAB), Abomey-Calavi, Benin
  • Marc Napoléon Assogba University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Issaka Youssao Abdou Karim University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Souaïbou Farougou University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin

Keywords:

Rhipicephalus (Boophilus) microplus, Cattle, Vegetable oil, DL50, DL90, Acaricidal, Benin

Abstract

The objective of this work was to study the acaricide activity of oils extracted from kernels of T. peruviana and A. muricata with the hexane at different concentrations on Rhipicephalus (Boophilus) microplus larvae aged from 14 to 21 days. For this purpose, the Larva Immersion Test was performed. Twice dilutions of both oils were tested from a starting dilution of 40% to 2.5%. There were three repetitions for each concentration and for each oil. The control solution in which these larvae were treated consisted of Tween-20, diluted at 2% in distilled water. The experimental groups were stored in an incubator at 27 ± 1 °C and at 85-90% of relative humidity for 24 hours. The larvae mortality was greater than 55% in all tested groups and reached 100% at the 40% concentration for A. muricata, while the control group had a 0% mortality. The LC50 and CL90 obtained were respectively 4.0331% and 10.7594% for A. muricata and 7.0942% and 41.4247% respectively for T. peruviana. These results clearly indicate that these oils all have acaricidal effects on Rhipicephalus (Boophilus) microplus larvae. However, A. muricata oil is the most toxic. It can therefore be used as an effective alternative to control the Rhipicephalus (Boophilus) microplus tick and there is a high probability that it can be used for other ticks affecting cattle and even other ectoparasites in Benin and worldwide, thereby reducing the use of synthetic acaricides which are toxic to the environment and ineffective against this tick.

References

Abdel-Shafy, S., Zayed, A.A., 2002. In vitro acaricidal effect of plant extract of neem seed oil (Azadirachta indica) on egg, immature, and adult stages of Hyalomma anatolicum excavatum (Ixodoidea: Ixodidae). Vet. Parasitol., 106(1), 89-96.

Adinci, K.J., Yessinou, R.E., Adehan, S.B., Yovo, M., Ahounou, S., Sessou, P., Adoligbé, C., Dossou, P.F., Avocegamou, S.Y., Youssao, A.K.I., Assogba, M.N., Mensah, G.A., Farougou, S., 2015. In vitro evaluation of the acaricidal effect of three essential oils extracted from aromatic local plants on Rhipicephalus (Boophilus) microplus ticks in Benin. Int. J. Adv. Res., 3(11), 1514-1520.

Atte, I., 1992. Enquête épidémiologique sur la sensisbilité des tiques aux acaricides au Niger: Cas des tiques du genre Hyalomma KOCH 1844. Thès. Doct. E.I.S.M.V. N°30 ; 111p.

Barros-Battesti, D.M., Arzua, M., Bechara, G.H., 2006. Carrapatos de Importância Médico-Veterinária da Região Neotropical: Umguiailustrado para identificação de espécies. São Paulo: Vox/ICTTD-3/Butantan, 223p.

Benavides, E., Romero, A., 2002. Consideraciones para el control integral de parásitosexternosdelganado. Carta Fedegan, Edición Nª 70, pps 1-7 (Disponible en internet http://www fedegan.org.co/70manual1 htm).

Bisen, S., Mandal, S.C., Sanyal, P.K., Pal, S., Sarkar, A.K., Patel, N.K., 2009. Efficacy (In vitro) of some Phytotherapeutic agents against Boophilus microplus. Proceeding of XIX National Congress of Veterinary Parasitology & National.

Champy, P., 2004b. Étude chimique et biologique des neurotoxines d’A. muricata L. (Annonaceae) pouvant être impliquées dans le survenue des parkinsonismes atypiques en Guadeloupe. Thèse de Doctorat de l'Université Paris-Sud 11.

Chougourou, 2012. Effets répulsifs des huiles extraites des plants locales Béninoises sur M. domestica.Countrystat/Bénin, 2015: Base de données statistiques. J. Appl. Biosci., http://countrystat.org/ home.aspx?c=BEN&tr=7

Dantas-Torres, F., 2008b. The brown dog tick, Rhipicephalussanguineus (Latreille, 1806) (Acari: Ixodidae): From taxonomy to control. Vet. Parasitol., 152, 173-185.

Dantas-Torres, F., 2010. Biology and ecology of the brown dog tick, Rhipicephalussanguineus. Parasit. Vector., 3, 26-37.

Davey, R.B., Miller, R.J., George, J.E., 2008. Efficacy of amitraz applied as a dip against an amitraz-resistant strain of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) infested on cattle. Vet. Parasitol., 152, 127-135.

Duarte, E.R., 2014. Extrato alcoólico de Capim-cidreira (Cymbopogon citratus) no controle do Boophilus Microplus Em Bovinos. Rev. Bras. Parasitol. Vet., 15, 37-39.

Eric, Y.R., Akpo, Y., Adoligbe, C., Adinci, J., Assogba, M.N., Koutinhouin, B., Abdou Karim, I.Y., Farougou, S., 2016b. Resistance of tick Rhipicephalus microplus to acaricides and control strategies. J. Entomol. Zool. Stud., 4(6), 408-414.

FAO, 2003. Resistencia a los antiparasitarios. Estadoactual con énfasis en América Latina. Dirección de Producción y Salud Animal. Roma, Italia. 52p.

Farias, M.P.O., Sousa, D.P., Arruda, A.C., Arruda, M.S.P., Wanderley, A.G., Alves, L.C., Faustino, M.A.G., 2007. Eficácia in vitro do óleo da Carapa guianensis Aubl. (Andiroba) no controle de Boophilus microplus (Acari: Ixodidae). Rev. Bras. Plant. Med. Botucatu., 9, 68-71.

Farias, M.P.O., Sousa, D.P., Arruda, A.C., Wanderley, A.G., Teixeira, W.C., Alves, L.C., Faustino, M.A.G., 2009. Potencial acaricida do óleo de andiroba Carapa guianensis Aubl. Sobre fêmeas adultas ingurgitadas de Anocentornitens Neumann, 1987e Rhipicephalus sanguineus Latreille, 1806. Arq. Bras. Med. Vet. Zootec., 61, 877-882.

Farougou, S., Adakal, H., Boko, C., 2013. Evaluation de la prévalence de la cowdriose chez les ruminants domestiques au Bénin. Rev. Méd. Vét., 12, 572-576.

Fourie, J.J., Ollagnier, C., Beugnet, F., Luus, H.G., Jongejan, F., 2013a. Prevention of transmission of Ehrlichiacanis by Rhipicephalus sanguineus ticks to dogs treated with a combination of fipronil, amitraz and (S)-methoprene. Vet. Parasitol., 193, 223-228.

Furlong, J., Martins, J.R., Prata, M.C.A., 2007. O carrapato dos bovinos e a resistência: Temos o que comemorar? Hora. Vet., 27, 26-32.

Ibelli, A.M., Ribeiro, A.R., Giglioti, R., Regitano, L.C., Alencar, M.M., Chagas, A.C., Paço, A.L., Oliveira, H.N., Duarte, J.M., Oliveira, M.C., 2012. Resistance of cattle of various genetic groups to the tick Rhipicephalus microplus and the relationship with coat traits. Vet. Parasitol., 186(3-4), 425-430.

Karimou, M., 1990. Méthodologie en évaluation de la sensibilité des tiques aux acaricides organophosphorés. Méd. Vét., N° 10, DAKAR.

Kearney, 2013. Acaricide resistance in cattle ticks. Northern Territory Government, (K58), 1-4.

Koch, H.G., 1982. Oviposition of the brown dog tick (Acari: Ixodidae) in the laboratory. Ann. Entomol. Soc. Am., 75, 583-586.

Lovis, L., Guerrero, F.D., Miller, R.J., Bodine, D.M., Betschart, B., Sager, H., 2012. Distribution patterns of three sodium channel mutations associated with pyrethroid resistance in Rhipicephalus (Boophilus) microplus populations from North and South America, South Africa and Australia. Int. J. Parasitol. Drug. Resist., 3(2), 216-24.

Maykelin Fuentes Zaldivar, 2014. Efecto del aceite del fruto de jatropha curcas en diferentes fases del ciclo biolo (Boophilus) microplus (Acari: Lxodidae). Gico de Rhipicephalus. Tesis en opción al Título de Master en Medicina Preventiva Veterinaria. Universidad Agraria de la Habana Fructuoso Rodriguez Perez Facultadde Medicina Veterinaria.

Miko, I., 1985. Dynamique saisonnière des tiques en zone sahélienne (région de Toukounous (Niger) relations avec les hôtes vertébrés domestiques et sauvages. Th. Sc., Paris Sud, 189p.

Pereira, J.R., 2006. In vitro efficacy of comercial formulations of ixocidides in engorged female of Boophilus microplus collected of dairy cattle at Paraíba Valey in the state of São Paulo. Rev. Bras. Parasitol. Vet., 15, 45-48.

R Core Team, 2015. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL http://www.R-project.org.

Rodriguez-Vivas, R.I., Mata, M.Y., Pe´ rez, G.E., Wagner, W., 2004. The effect of management factors on the seroprevalence of Anaplasma marginale in Bos indicus cattle in the Mexican tropics. Trop. Anim. Health. Prod., 36, 135-143.

Roel, A.R., 2001. Utilização de plantas com propriedades inseticidas: Umacontribuição para o desenvolvimento rural sustentável. Revista Internacional de Desenvolvimento Local, 1(2), 43-50.

Rosado-Aguilar, J.A., Aguilar, C.A., Rodriguez-Vivas, R.I., Borges, A.R., Garcia-Vazquez, Z., Mendez-Gonzalez, M., 2010. Acaricidal activity of extracts from Petiveria alliacea (Phytolaccaceae) against the cattle tick, Rhipicephalus (Boophilus) microplus (Acari: ixodidae). Vet. Parasitol., 168(3-4), 299-303.

Saiyed, H., Dewan, A., Bhatnagar, V., Shenoy, U., Shenoy, R., Rajmohan, H., Patel, K., Kashyap, R., Kulkarni, P., Rajan, B., 2003. Effect of endosulfan on male reproductive development. Environ. Health. Perspect., 111, 1958-1962.

Shyma, K.P., Gupta, J.P., Ghosh, S., Patel, K.K., Singh, V., 2014. Acaricidal effect of herbal extracts against cattle tick Rhipicephalus (Boophilus) microplus using in vitro studies. Parasitol. Res., 113, 1919.

Silva, W.C., Martins, J.R.S., Souza, H.E.M., Heinzen, H., Cesio, M.V., Mato, M., Albrecht, F., Azevedo, J.L., Barros, N.M., 2009. Toxicity of Piper aduncum L. (Piperales: Piperaceae) from the amazon forest for the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Vet. Parasitol., 164, 267-274.

Soberanes, C.N., Santamaria, V.M., Fragoso, S.H., Garcia, V.Z., 2002. Primer caso de resistencia al amitraz en la garrapata del ganado Boophilus microplusen Mexico. Tec. Pec. Mex., 40, 81-92.

Srivastava, R., Ghosh, S., Mandal, D.B., Azhahianambi, P., Singhal, P.S., Pandey, N.N., 2008. Efficacy of Azadirachta indica extracts against Boophilus microplus. Parasitol. Res., 104(1), 149-53.

Yessinou, R.E., Adinci, J., Sessou, P., Adehan, S., Tonouweha, A., Akpo, Y., Adoligbe, C., Assogba, M.N., Koutinhouin, B., Youssao, A.K.I., Farougou, S., 2016a. In vitro acaricidal effect of Syzygium aromaticum and Cymbopogon citratus essential oil on engorged female of cattle tick Rhipicephalus microplus in Benin. Sci. J. Vet. Adv., 5(3), 80-86.

Downloads

Published

2017-03-22

How to Cite

Justin Adinci, K. ., Akpo, Y. ., Tonouhewa, A. ., Eric Yessinou, R. ., Sessou, P. ., Yovo, M. ., Bienvenu Adehan, S. ., Adoligbe, C. ., Appolinaire Mensah, G. ., Napoléon Assogba, M. ., Abdou Karim, I. Y. ., & Farougou, S. . (2017). In vitro evaluation of the acaricidal effect of vegetal oils extracted from the kernel of Thevetia peruviana and Annona muricata on the Rhipicephalus (Boophilus) microplus larvae. Scientific Journal of Veterinary Advances, 6(3), 162-169. Retrieved from http://sjournals.com/index.php/sjva/article/view/240

Issue

Vol. 6 No. 3 (2017): March

Section

Original Article

License

Copyright (c) 2020 Kossi Justin Adinci, Yao Akpo, Arétas Tonouhewa, Roland Eric Yessinou, Philippe Sessou, Mahuto Yovo, Safiou Bienvenu Adehan, Camus Adoligbe, Guy Appolinaire Mensah, Marc Napoléon Assogba, Issaka Youssao Abdou Karim, Souaïbou Farougou

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Most read articles by the same author(s)