TEL-AML-1 fusion gene in children with acute lymphoblastic in basra pediatric oncology center
Keywords:
Acute lymphoblastic leukemia, TEL_AML1, fusion geneAbstract
TEL-AML-1 fusion gene resulting from 12,21 chromosomal translocation is believed to be the most common molecular genetic abnormality in childhood acute lymphoblastic leukemia(ALL). This study has been conducted to investigate the frequency of this fusion gene in children suffering from ALL attending the oncology unit in Basra hospital for pediatric and gynecology during the period from May 2009 to April 2010, and point out the different laboratory features associated with this anomalies. A total of 120 blood samples were collected( 60 early diagnosed all children and 60 healthy children as control group). The controls were matched with cases by age and sex. Ribonucleic acid (RNA) has been successfully extracted from 40 ALL cases fresh blood used for the detection of TEL-AML-1 fusion gene by reverse transcriptase- polymerase chain reaction (RTPCR). Of newly diagnosed all cases 27.5% were positive for TEL-AML- 1 fusion gene as well as 5% among the control group. All TEL-AML-1 positive cases showed an age peak between 3-6 years and tend to occur more frequent among female than males. TEL-AML-1 positive cases that classified as standard risk group were accounted for 72.3% while 27.3% were high risk group (P<0.05) and according to the French- -American-British (FAB) classification criteria, 72.3% of high risk and 68% of standard risk groups belong to L2 stage. TEL-AML-1 fusion gene identifies a subset of pediatric ALL associated with a number of laboratory markers of good prognosis and should thus be considered in routine molecular work of ALL to confirm its impact on clinical outcome and to design suitable therapy.
References
Andersen, M.T., Nordentoft, I., Hjalgrim, L.L., 2001. Characterization of t(12,21) breakpoint junctions in acute
lymphoblastic leukemia. Nature., 15 (5), 858-859 .
Anthony M.F., Karin F., Renate P.E., Origine of "late" relapse in childhood lymphoblastic leukemia with TEL-AML-1
fusion genes. Blood, 98(3), 558-564.
Anthony, M.F., Chiara, P., Clara, B., 2009. The TEL-AML-1 leukemia fusion gene dysregulates the TGE-B pathway in
early B lineage progenitor cells. J. Clin. Invest., 119(4), 826-836.
Armstrong, S.A., Look, T.A., 2005. Molecular genetics of acute lymphoblastic leukemia. J. Clin. Oncol., (Rev Article),
(26), 6306-6315.
Arthur, Z., Mel, G., Tariq, E., 2004. Role of TEL-AML-1 fusion gene in the molecular pathogenesis of childhood
acute lymphoblastic leukemia. Oncogene., 23, 4275-4283.
Artigas A, Carmen G, 2006. Frequency of TEL-AML-1 and BCR-ABL fusion genes in children with acute lymphoblastic
leukemia. Rev Ned Chile(online), 134(11), 1367-1376.
Cave, H., Cacheux, V., Raynaud, S., 1997. ETV6 is the target of chromosome 12p deletion in t(12,21) childhood
acute lymphoblastic leukemia. Leukemia., 11, 1459-1464.
Charles, G.M., Salil, G., Ina, R., 2007. Genome-wide analysis of genetic alteration in acute lymphoblastic leukemia.
Nature 446,758-764.
Eguchi-Ishimae, M., Eguchi, M., Tanaka, K., 1998. Fluorescence insitu hybridization analysis of 12;21 translocation
in Japanese childhood acute lymphoblastic leukemia . Japn. J. Cancer. Res., 89, 783-788.
Einav, U., 2003. Class discovery in acute lymphoblastic leukemia using gene expression analysis. MSc thesis,
Scientific council of the Weizmann.
Fabio, T., Maurizio, A., 2008. Treatment of pediatric acute lymphoblastic leukemia. haematolog., 93(8), 1124-
Fatih, M.U., Niels, P.b., Peter, H., Christopher, N., 2001. Expression of TEL-AML-1 fusion transcript and response to
induction therapy in standard risk acute lymphoblastic leukemia. Leukem. Lymphoma., 42(1&2), 41-56.
Garcia-Sanz, R., Alaejos, I., Orfao, A., 1999. Low frequency bof the TEL-AML-1 fusion gene in acute lymphoblastic
leukemia in Spain. Br. J. Hematol., 107, 667-669.
Gary, G.D., 2001. Origin and clinical significance of the TEL-AML-1 fusion. Blood 97 (3), 73.
Greaves, M., 1997. Aetiology of acute lymphoblastic leukemia. Lancet 349,344-349.
Harbott, J., Viehmann, S., Borkhardt, A., 1997. Incidence of TEL-AML-1 fusion gene analysed consecutively in
children with acute lymphoblastic leukemia in relapse. Blood., 90, 4933-4937.
Inamdar, N., Kumar, S.A., Banavali, S.D., 1998. Comparative incidence of the rearrangement of TEL-AML-1 and ALL1
gene in pediatric precursor B acute lymphoblastic leukemia in India. Int. J. Oncol., 13, 1319-1322.
Jaro-ova, M., Holzerova, M., Mihal, V., 2002. Additional evidence of genetic changes in children with ALL and TELAML-1 fusion gene. Leukemia., 16 , 1873-1875.
Jim, V.D., Macintyre, E.A., Gabert, A., 1999. Standardized RT-PCR analysis of fusion gene transcript from
chromosome aberrations in acute leukemia for detection of minimal residual disease. Leukemia., 13,1901-
Jukka K., 2001. Prognostic factors in childhood acute lymphoblastic leukemia (ALL) , PhD thesis submitted to
Haartman Institute University of Helsinki, Finland.
Kazunori, N., Mary, S., Kenneth, B., 2006. Age difference in immunophenotype of acute leukemia. Am. J.
Immunol., 2(3), 64-70
Magalhaes, I.G., Pombo-de-Oliveira, M.S., Bennett, C.A., 2000. TEL-AML-1 fusion gene frequency in pediatric acute
lymphoblastic leukemia in Brail. Br. J. Hematol., 11(1), 204-207.
Marks, D., Kurz, B.W., Link, M.P., 1996. High incidence of potential P53 inactivation in poor outcome childhood
acute lymphoblastic leukemia at diagnosis . Blood., 87(3), 1155-1161.
McLean, T.W., Ringold, S., Neuberg, D., 1996. TEL-AML-1 dimerizes and is associated with a favourable outcome
in childhood acute lymphoblastic leukemia. Blood, 88, 4252-4258.
Mosad, E., Hamed, B.H., Bakry, M.R., 2008. persistence of TEL-AML-1 fusion gene as minimal residual disease has
no additive prognostic value in CD10 positive B-acute lymphoblastic leukemia, a FISH study. J Hematol
Oncology, 1 , 17 (available at http,//www.jhoonline.org/content/1/ 1/17
Nakao, M., Yokota, S., Horiike, S., 1996. Detection and quantification of TEL-AML-1 fusion transcripts by
polymerase chain reaction in childhood acute lymphoblastic leukemia. Leukemia., 10, 1463-1470.
Nina, A., 2006. P53 protein biosignitures in acute myeloid leukemia. MSc thesis, University of Bergen.
Petridou, E., Dalamaga, M., Mentis, A., 2001. Evidence on the infectious aetiology of childhood leukemia, the role
of low herd immunity(Greece). Cancer Causes Control., 12 , 645-652.
Pui, C.H., 1995. Childhood leukemia. N. Engl. J. Med., 332,1618-1630.
Roumier, C., Fenaux, P., Lafage, M., 2003. New mechanisms of AML-1 gene alteration in hematol. Malignanc.
Leukemia., 17, 9-16.
Rubnitz, J.E., Downing, J.R., Pui, C.H., 1997. TEL gene rearrangement in acute lymphoblastic leukemia, a new
genetic marker with prognostic significance . J. Clin. Oncol., 15 , 1150-1157 .
Rubnitz, J.E., Pui, C.H., Downing, J.R., 1999. The role of TEL fusion gene in pediatric leukemia. Leukemia., 13(1), 6-
(ISSN, 0887-6924).
Scott, A.A., Look, A.T., 2005. Molecular genetics of acute lymphoblastic leukemia. J. Clin. Oncol., 23, 26
Shaker, H.M., Sidhom, I.A., El-Attar, I.A., 2001. Frequency and clinical relevance of TEL-AML-1 fusion gene in
childhood acute lymphoblastic leukemia in Egypt . J. Egypt. Nat. Cancer Instit., 13(1), 9-18.
Spathas, D.H., Stewart, J., Singer, IO, et al. Detection, T., 1999. (12,21) in childhood lymphoblastic leukemia by
fluorescence instu hybridization. Cancer Genel Cytogenet., 110, 7-13.
William, L.C., Deepa, B., Dong-Joon, M., 2003. Pediatric acute lymphoblastic leukemia. Am. Soc. Hematol., 29(2),
-50.
Zuna, J., Hrusak, O., Kalinova, M., 1999. TEL-AML-1 positivity in childhood ALL, average or better prognosis? Czech
Pediat Hematol Working Group. Leukemia., 13 , 22-24 .
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