Scientific Journal of Microbiology https://sjournals.com/index.php/sjm <p>Scientific Journal of Microbiology (SJM) is an international peer-reviewed journal dedicated to publish the results of original research in shape of original articles, reviews and short communications. Scope of the journal includes:fungi and other unicellular eukaryotic organisms. Topics include structure and function, biochemistry, enzymology, metabolism and its regulation, molecular biology, genetics, plasmids and transposons, general microbiology, applied microbiology, genetic engineering, virology, immunology, clinical microbiology, microbial ecology, environmental microbiology, food microbiology, molecular systematics, chemical or physical haracterization of microbial structures or products, and basic biological properties of organisms.</p> en-US onlinesjournals@gmail.com (Executive Managing Editor) onlinesjournals@gmail.com (Farhad Jazideh) Wed, 10 Mar 2021 00:00:00 +0100 OJS 3.2.0.3 http://blogs.law.harvard.edu/tech/rss 60 Molecular characterization of potential allergenic molds (Aspergillus, Penicillium and Fusarium) in two buildings receiving the public in Abidjan – Côte d’Ivoire https://sjournals.com/index.php/sjm/article/view/1651 <p><em>Aspergillus, Penicillium </em>and<em> Fusarium </em>species play an important role in environmental allergy. These molds are potential density in the indoor environment. This study aimed to characterize the strains <em>Aspergillus, Penicillium </em>and<em> Fusarium </em>using a molecular method. The beta-tubulin and elongation factor TEF- genes were utilized to differentiate the species. A total of 405 environmental species (<em>Aspergillus, Penicillium </em>and<em> Fusarium</em>) were isolated, purified and initially identified by colony morphology. Subsequently, DNA was extracted and; PCR was performed from 351 isolates. The results were then compared to morphological characteristics. Of the 351 isolates tested, 167 were <em>Aspergillus</em>, 103 were <em>Penicillium</em> and 81 were <em>Fusarium</em>. The beta-tubulin and elongation factor TEF genes were found to be the most suitable for differentiating these three genera among them; the beta-tubulin gene was used for molecular identification of <em>Aspergillus, Penicillium</em> and the elongation factor TEF gene for characterizing <em>Fusarium</em> species.</p> M’boh Epi Reine Elisabeth N’Gou, David Coulibaly N’Golo, Chiaye Claire Antoinette Yapo-crezoit, Ama Valerie Bonouman, Solange Kakou, Ossey Bernard Yapo, Allico Joseph Djaman, Mireille Dosso Copyright (c) 2021 M’boh Epi Reine Elisabeth N’Gou, David Coulibaly N’Golo, Chiaye Claire Antoinette Yapo-crezoit, Ama Valerie Bonouman, Solange Kakou, Ossey Bernard Yapo, Allico Joseph Djaman, Mireille Dosso https://creativecommons.org/licenses/by-nc-nd/4.0 https://sjournals.com/index.php/sjm/article/view/1651 Mon, 29 Mar 2021 00:00:00 +0200