https://sjournals.com/index.php/sjm/issue/feed Scientific Journal of Microbiology 2021-09-10T01:59:47+02:00 Executive Managing Editor onlinesjournals@gmail.com Open Journal Systems <p>Scientific Journal of Microbiology (SJM) is an international peer-reviewed journal dedicated to publish the results of original research in shape of original articles, reviews and short communications. Scope of the journal includes:fungi and other unicellular eukaryotic organisms. Topics include structure and function, biochemistry, enzymology, metabolism and its regulation, molecular biology, genetics, plasmids and transposons, general microbiology, applied microbiology, genetic engineering, virology, immunology, clinical microbiology, microbial ecology, environmental microbiology, food microbiology, molecular systematics, chemical or physical haracterization of microbial structures or products, and basic biological properties of organisms.</p> https://sjournals.com/index.php/sjm/article/view/1651 Molecular characterization of potential allergenic molds (Aspergillus, Penicillium and Fusarium) in two buildings receiving the public in Abidjan – Côte d’Ivoire 2021-09-10T01:59:47+02:00 M’boh Epi Reine Elisabeth N’Gou reinemboh@hotmail.com David Coulibaly N’Golo reinemboh@hotmail.com Chiaye Claire Antoinette Yapo-crezoit reinemboh@hotmail.com Ama Valerie Bonouman reinemboh@hotmail.com Solange Kakou reinemboh@hotmail.com Ossey Bernard Yapo reinemboh@hotmail.com Allico Joseph Djaman reinemboh@hotmail.com Mireille Dosso reinemboh@hotmail.com <p><em>Aspergillus, Penicillium </em>and<em> Fusarium </em>species play an important role in environmental allergy. These molds are potential density in the indoor environment. This study aimed to characterize the strains <em>Aspergillus, Penicillium </em>and<em> Fusarium </em>using a molecular method. The beta-tubulin and elongation factor TEF- genes were utilized to differentiate the species. A total of 405 environmental species (<em>Aspergillus, Penicillium </em>and<em> Fusarium</em>) were isolated, purified and initially identified by colony morphology. Subsequently, DNA was extracted and; PCR was performed from 351 isolates. The results were then compared to morphological characteristics. Of the 351 isolates tested, 167 were <em>Aspergillus</em>, 103 were <em>Penicillium</em> and 81 were <em>Fusarium</em>. The beta-tubulin and elongation factor TEF genes were found to be the most suitable for differentiating these three genera among them; the beta-tubulin gene was used for molecular identification of <em>Aspergillus, Penicillium</em> and the elongation factor TEF gene for characterizing <em>Fusarium</em> species.</p> 2021-03-29T00:00:00+02:00 Copyright (c) 2021 M’boh Epi Reine Elisabeth N’Gou, David Coulibaly N’Golo, Chiaye Claire Antoinette Yapo-crezoit, Ama Valerie Bonouman, Solange Kakou, Ossey Bernard Yapo, Allico Joseph Djaman, Mireille Dosso