TY - JOUR AU - Sarker, Ashish Kumar AU - Haque, Anwarul AU - Islam, Mohammad Sayful AU - Haque, Uzzal AU - Kundo, Netish Kumar AU - Anisuzzaman, Abu Syed AU - Saha, Urmi AU - Islam, Anwar Ul PY - 2015/09/30 Y2 - 2024/03/28 TI - Phenotypic and genotypic characterization of a marine bacterium isolated from Sundarbans, Bangladesh JF - Scientific Journal of Microbiology JA - Sci. J. Microbiol. VL - 4 IS - 7 SE - DO - UR - https://sjournals.com/index.php/sjm/article/view/395 SP - 58-69 AB - <p>The mangrove ecosystem is a largely unexplored source for bacteria with potentiality to produce biologically active secondary metabolites. Consequently, we set our research study to isolate, characterize and screen a bacterium producing bioactive compounds from marine soil of largest mangrove forest of the world, Sundarbans, Bangladesh. A total number of 39 marine bacterial colonies were isolated and purified. Representative bioactive isolate, ANAM-5 was characterized using phenotypic and genotypic procedures since it exhihibited highest antibacterial activity against a series of test organisms. The 16S rDNA sequence of the strain ANAM-5 showed close similarity with only one bacterium Planctomyces&nbsp;<em>brasiliensis</em>, complete genome (99.64%). But the cultural, morphological, physiological and biochemical characteristics of the strain and P.&nbsp;<em>brasiliensis</em>&nbsp;were completely different. So, it may not be logical to assign the strain ANAM-5 to the genus Planctomyces. Thus, to confirm the taxonomic position of the strain and to have a correct solution from this taxonomic problem, further studies is needed in respect to the DNA relatedness studies, fatty acid profiling, DNA-DNA hybridization, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), metabolic finger profiling using BIOLOG, ribotyping, small subunit (SSU) sequences, cell wall composition and other characterizations.</p> ER -