TY - JOUR AU - Nazari, Zahra AU - Amini, Kumarss AU - Mokhtari, Alireza PY - 2015/09/29 Y2 - 2024/03/28 TI - Detection of diarrhoeagenic Escherichia coli virulence genes by multiplex-PCR method and their antibiotic susceptibility profile JF - Scientific Journal of Microbiology JA - Sci. J. Microbiol. VL - 4 IS - 7 SE - DO - UR - https://sjournals.com/index.php/sjm/article/view/393 SP - 40-48 AB - <div><p>Acute&nbsp; diarrhoeal&nbsp; disease&nbsp; is&nbsp; a&nbsp; public&nbsp; health&nbsp; problem&nbsp; and&nbsp; an&nbsp; important cause of morbidity and mortality, particularly in&nbsp; developing countries. The etiology is varied, and the diarrheagenic Escherichiacolipathotypes are most important.&nbsp; Our objectives were detection of Diarrhoeagenic&nbsp;<em>E. coli</em>&nbsp;virulence genes by Multiplex-PCR method and their antimicrobial susceptibility profile. Fifty five&nbsp;<em>E.&nbsp; Coli</em>&nbsp;strains were isolated from 150 fresh fecal samples. Multiplex PCR was performed to characterize the diarrheagenic&nbsp;<em>E. coli</em>&nbsp;strains. Antibiotic susceptibility patterns were determined by the disc-diffusion method. The highest and lowest frequency&nbsp; of&nbsp; DEC&nbsp; isolates&nbsp; were&nbsp; 12.7%&nbsp; and&nbsp; 1.8%&nbsp; for&nbsp; EHEC&nbsp; and&nbsp; EIEC,&nbsp; respectively. bfp&nbsp; and&nbsp; stx1&nbsp; genes&nbsp; were&nbsp; not&nbsp; carried&nbsp; out&nbsp; by&nbsp; DEC strains. Thirty six isolates were negative for any gene amplification. The highest rate of&nbsp; resistance&nbsp; among&nbsp; DEC&nbsp; strains&nbsp; were&nbsp; related&nbsp; to&nbsp; Imipenem with&nbsp; 100% frequency.&nbsp; All&nbsp; DEC&nbsp; isolates&nbsp; (100%)&nbsp; were&nbsp; susceptible&nbsp; to&nbsp; ciprofloxacin.Differentiation between the diarrheagenic&nbsp;<em>E. coli</em>&nbsp;pathotypes&nbsp; are&nbsp; of&nbsp; great&nbsp; importance&nbsp; since&nbsp; they&nbsp; are&nbsp; complicated&nbsp; in&nbsp; acute&nbsp; diarrheal&nbsp; illnesses&nbsp; and&nbsp; may&nbsp; need&nbsp; specific&nbsp; antimicrobial&nbsp; therapy. The high antimicrobial resistance raises a broad discussion on the indiscriminate or improper use of antimicrobials, besides the risks of self-medication.</p></div> ER -