Molecular characterization of potential allergenic molds (Aspergillus, Penicillium and Fusarium) in two buildings receiving the public in Abidjan – Côte d’Ivoire

Authors

  • M’boh Epi Reine Elisabeth N’Gou Institut Pasteur of Côte d’Ivoire (Biology of Immunity Unit, Molecular Biology Platform, Mycology Laboratory, Biochemistry Laboratory, Microbiology Laboratory).
  • David Coulibaly N’Golo Institut Pasteur of Côte d’Ivoire (Biology of Immunity Unit, Molecular Biology Platform, Mycology Laboratory, Biochemistry Laboratory, Microbiology Laboratory).
  • Chiaye Claire Antoinette Yapo-crezoit Institut Pasteur of Côte d’Ivoire (Biology of Immunity Unit, Molecular Biology Platform, Mycology Laboratory, Biochemistry Laboratory, Microbiology Laboratory).
  • Ama Valerie Bonouman Institut Pasteur of Côte d’Ivoire (Biology of Immunity Unit, Molecular Biology Platform, Mycology Laboratory, Biochemistry Laboratory, Microbiology Laboratory).
  • Solange Kakou Institut Pasteur of Côte d’Ivoire (Biology of Immunity Unit, Molecular Biology Platform, Mycology Laboratory, Biochemistry Laboratory, Microbiology Laboratory).
  • Ossey Bernard Yapo Central Environmental Laboratory, Ivorian Anti-Pollution Centre (CIAPOL).
  • Allico Joseph Djaman Université Félix Houphouët Boigny of Côte d’Ivoire (Biology and Health Laboratory), 22 BP 582 Abidjan 22.
  • Mireille Dosso Institut Pasteur of Côte d’Ivoire (Biology of Immunity Unit, Molecular Biology Platform, Mycology Laboratory, Biochemistry Laboratory, Microbiology Laboratory).

Keywords:

Allergy, Moulds, Aspergillus, Penicillium, Fusarium, PCR.

Abstract

Aspergillus, Penicillium and Fusarium species play an important role in environmental allergy. These molds are potential density in the indoor environment. This study aimed to characterize the strains Aspergillus, Penicillium and Fusarium using a molecular method. The beta-tubulin and elongation factor TEF- genes were utilized to differentiate the species. A total of 405 environmental species (Aspergillus, Penicillium and Fusarium) were isolated, purified and initially identified by colony morphology. Subsequently, DNA was extracted and; PCR was performed from 351 isolates. The results were then compared to morphological characteristics. Of the 351 isolates tested, 167 were Aspergillus, 103 were Penicillium and 81 were Fusarium. The beta-tubulin and elongation factor TEF genes were found to be the most suitable for differentiating these three genera among them; the beta-tubulin gene was used for molecular identification of Aspergillus, Penicillium and the elongation factor TEF gene for characterizing Fusarium species.

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Published

2021-03-29

How to Cite

N’Gou, M. E. R. E. ., N’Golo, D. C. ., Yapo-crezoit, C. C. A. ., Bonouman, A. V. ., Kakou, S. ., Yapo, O. B. ., Djaman, A. J. ., & Dosso, M. . (2021). Molecular characterization of potential allergenic molds (Aspergillus, Penicillium and Fusarium) in two buildings receiving the public in Abidjan – Côte d’Ivoire. Scientific Journal of Microbiology, 7(2). Retrieved from https://sjournals.com/index.php/sjm/article/view/1651

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Original Article