Evaluation of protein, monosaccharide, free proline content during grain filling in Triticum Aestivum V. Zarrin

Authors

  • H. Masoud Department of Biological science and Biotechnology, Faculty of Basic science, University of Kurdistan, Iran
  • A. Nesa Department of Biological science and Biotechnology, Faculty of Basic science, University of Kurdistan, Iran

Keywords:

Grain filling;Wheat;Protein content;Free proline;Stress condition

Abstract

The progress of seed development was accompanied change inpattern of biochemical and physiological parameter which affected byenvironmental condition and developmental stage during grain filling period. Weperformed several studies to understand the biochemical and physiologicalevents taking place during seed grain filling. In this investigation, westudied changes in protein, monosaccharide and free   prolinecontent as biochemical parameter and fresh weight   as a growth parameter during grain filling(8weeks) period at field growing  Iranianwheat cultivar ( Triticum aestivum v. zarrin) . Results indicate that the mostincrease in fresh weight occurs between first and second week after anthesis.Constant phase of weight change occurred in 2, 3 and 4 week age. Significantdecrease in fresh weight occurs in 5 week age of grain filling. Increase inprotein content at 6 week age of grain may be related to biosynthesis oflate-embryogenesis-abundant (LEA) proteins that thought to be involved indesiccation during dehydration phase of seed development in wheat grain notsimilar to stress condition.

References

Bates L.S., 1973. Rapid determination of free prolin for water sterss studies. plant soil., 39.205-207.

Black, et al., 1999. Black, M., Bradford, K., Ramos, J., 1999. Seed biol. Ad. appl., 27-41 pp.508.CABI Publishing,UK.

Bollage, D.M., Edelstine, S.J., 1990. Protein methods.Wiley-Uss.pp., 543-583.

Buchanan,B.B., Grussemand, W., Jones, L., 2000. Biochemistry&MolecuarBiology of plants.Amer. Soc. plant Physiologist, Rokville. Maryland. pp., 1158-1204.

Chaplin,,M.F., kenneedy, J.F., 1987. Carbohydrate analysis.IRLpress.oxford.Washington., DC.pp. 228.

Cornell, H.j., Hoveling, A.w., 1998. Wheat chemistery and utilization.T P company, Inc. Pennsylvania. U.S.A pp, 342-352.

Daniel, C., Triboi, E., 2002. Changes in Wheat protein aggregation during grain development.Eur. j. Agr., 16,1-12.

Dupont, F.M., Altenbach, S.B., 2003. Molecular and biochemical impacts of enviromental factors on wheat grain development and protein synthesis.j. cereal sci., 38,133-146.

Dubois, M., Gilles, K., Hamiltone, J.K., 1951., Acolorimetric methodfor determination of sugars.Nature.London., pp 168.

Finnie, et al., 2002. Finnie,Ch.;Melchior,s.2002.Proteome analysis of grain filling and seed maturation in barly.Plant physiol. Prev., June 14,2002;10.1104/pp.003681.

Gebbing, T., Schnyder, H., 1999. Pre-Anthesis reserve utilization for protein and carbohydrate Synthesise durng grain fillin. Plant physiol., vol.121, pp. 871-879.

Gooding, M., Ellist, T., 2003. Effect of Restricted water availability and increased temperature on the grain filling ,druing and quality of winter wheat. j. cereal sci., 37,295-309.

Haidari, R., Haidarizadeh, M., 2003. Effect of temprature stress on total protein and soluble sugars content in Iranian wheat cultivars.Iran. j. biol., vol.13,No.3,4. Pp, 59-68.

Hale, M.G., 1987. The physiology of plant s under sterss. John Wily sons. Inc.U.S.A.

Hoekstra, F.A., Golovina, E.A., 2000. Impact of amphiphile partiotioninig on desiccation tolrrance.Seed biol. adv. appl., pp.43-55. CABI publishing.UK.

Rosebrough, n.j., Farr,A.L., 1950. Randall. R. J. j. Biol. Chem., 193, 265.

Ross, C.W., 1974. Plantphysiology laboratory manual.wordworth publishing company INC., pp.200.

Sanchez, E.J., 2000. Protein synthesis in seed germination:conecting external cell signals with the ribosome. Seed biol. adv. appli., pp.11-24. CABI publishing.UK.

Sotres,R., Nunez,A., 2000. Possible roles of phosphoenol pyruvate carboxylase in assimilate partitioning in developing maize embryos. Seed biol. adv. appl., pp.141-151. CABI publishing.UK.

Taiz, L., Ziger.E., 1998. Plant physiology.Sinauer assocites. Inc.

eare, I., Ehler, S., 1974. Changes in free proline concentratio in sorghum and soybean plants under fild conditions.Crop. sci., vol.14, 447-450.

Published

2013-09-29

How to Cite

Masoud, H. ., & Nesa, A. . (2013). Evaluation of protein, monosaccharide, free proline content during grain filling in Triticum Aestivum V. Zarrin. Agricultural Advances, 2(9), 270-276. Retrieved from http://sjournals.com/index.php/aa/article/view/739

Issue

Section

Original Article