Phenotypic and genotypic characterization of a marine bacterium isolated from Sundarbans, Bangladesh
Keywords:
Phenotypic, Genotypic, Marine Bacteria, SundarbansAbstract
The mangrove ecosystem is a largely unexplored source for bacteria with potentiality to produce biologically active secondary metabolites. Consequently, we set our research study to isolate, characterize and screen a bacterium producing bioactive compounds from marine soil of largest mangrove forest of the world, Sundarbans, Bangladesh. A total number of 39 marine bacterial colonies were isolated and purified. Representative bioactive isolate, ANAM-5 was characterized using phenotypic and genotypic procedures since it exhihibited highest antibacterial activity against a series of test organisms. The 16S rDNA sequence of the strain ANAM-5 showed close similarity with only one bacterium Planctomyces brasiliensis, complete genome (99.64%). But the cultural, morphological, physiological and biochemical characteristics of the strain and P. brasiliensis were completely different. So, it may not be logical to assign the strain ANAM-5 to the genus Planctomyces. Thus, to confirm the taxonomic position of the strain and to have a correct solution from this taxonomic problem, further studies is needed in respect to the DNA relatedness studies, fatty acid profiling, DNA-DNA hybridization, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), metabolic finger profiling using BIOLOG, ribotyping, small subunit (SSU) sequences, cell wall composition and other characterizations.
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Copyright (c) 2020 Ashish Kumar Sarker, Anwarul Haque, Mohammad Sayful Islam, Uzzal Haque, Netish Kumar Kundo, Abu Syed Anisuzzaman, Urmi Saha, Anwar Ul Islam
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