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Evaluation of bovine oviduct epithelial cells culture in monolayer system

Shiva Erfani, Taher Harkinezhad, Hamid Amanlou

Abstract


The oviduct is an important site in reproduction, such as fertilization and early embryonic development. In this study bovine oviduct epithelial cells (BOEC) isolated and cultured in monolayer system to evaluate the proliferation and viability of cells in quality manner before and after two time passages. The epithelial cells isolated mechanically from oviduct tissue in sterile condition. Isolated cells floated in culture medium, fetal bovine serum and antibiotics in a T-24 culture flask. Two days after seeding have been observed that cells adhered to flask surface and are proliferating. The rate of cell proliferation was well. After one week the cells covered the flask surface and were confluent. Then the cells isolated from flask by trypsin-EDTA enzyme and passage into 4 flasks. Cells cover the flasks in 10 days and passage into 12 flasks. In the second passage the cells was confluent in 12 days. We observed that in first culture there was a little dead cell during the culture and cells proliferation rate was high. After each passage the number of dead cells in flasks increased and cells proliferation rate in each passage decreased.


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