Open Access Open Access  Restricted Access Subscription Access
Cover Image

Simple Test kit for Rapid Detection of the presence of Canine Parvovirus Antigen from Dogs Feces

Omar Abashar Al-Tayib

Abstract


Canine parvovirus (CPV) is one of the most important viral infection diseases infects all the dogs worldwide. The management and preventive of this disease is such important target. SensPERT® CPV antigens tests are available to detect parvovirus among dogs, and presumptively to predict protective status. The aim of this study was to determine the diagnostic accuracy of the test to detect the virus by using fecal samples collected from dogs housed at experimental laboratory animals, with or without gastroenteritis sings from Riyadh, Saudi Arabia. The dogs were divided into two groups on the basis of age, vaccination and clinical signs using SensPERT test kits. Prevalence to CPV infection in these dogs was significantly higher in hemorrhagic diarrheic dogs less than 3 months (84%; 21 positive of 25) by means of immunochromatography assay, followed by puppies without signs (40%; 4 positive of 10).Nevertheless, there were no infections in adult vaccinated dogs (100%; 15/15). SensPERT One-rapid test kit was demonstrated to be a useful, simple and very quick diagnostic tool for determining CPV status in dogs populations and it will giving a good idea regarding dogs vaccination importance to prevent and protects other animals against infection.


References


Appel, M.J., Scott, F., Carmichael, L., 1979. Isolation and immunisation studies of a canine parvolike virus from dogs with haemorrhagic enteritis. Vet. Rec., 105,156–159.

Buonavoglia, C., Martella, V., Pratelli, A., Tempesta, M., Cavalli, A., Buonavoglia, D., Bozzo, G., Elia, G., Decaro, N., Carmichael, L., 2001. Evidence for Evolution of Canine Parvovirus Type 2 in Italy. J. Gen.Virol., 82, 3021–3025.

Cavalli, A., Bozzo, G., Decaro, N., Tinelli, A., Aliberti, A., Buonavoglia, D., 2001. Characteriza

tion of a canine parvovirus strain isolated from an adult dog. New Microbiol., 24, 239–242.

Decaro, N., Desario, C., Campolo, C., Cavalli, A., Ricci, D., Martella, V., Tempesta, M., Buonavoglia, C., 2004. Evaluation of the lactogenic immunity to canine parvovirus in pups. New Microbiol., 27, 375–379.

Decaro, N., Campolo, M., Desario, C., Elia, G., Martella, V., Lorusso, E., Buonavoglia, C., 2005. Maternally-derived antibodies in pups and protection from canine parvovirus infection. Biologicals., 33, 259–265.

Decaro, N., Desario, C., Beall, M.J., Cavalli, A., Campolo, M., Dimarco, A., Amorisco, F., Colaianni, M.L., Buonavoglia, C., 2010. Detection of canineparvovirus type2c by a commercially available in-house rapid test. Vet. J., 184, 373–375.

Decaro, N., Buonavoglia, C., 2012. Canine parvovirus- A review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet. Microbiol., 155, 1–12.

de Castro, T.X., Uchoa, C.M., de Albuquerque, M.C., Labarthe, N.V., Garcia Rd, C.N., 2007. Canine parvovirus (CPV) and intestinal parasites: laboratorial diagnosis and clinical signs from puppies with gastroenteritis. Int. J. Appl. Res. Vet. Med., 5, 72–76.

Desario, C., Decaro, N., Campolo, M., Cavalli, A., Cirone, F., Elia, G., Martella, V., Lorusso, E., Camero, M., Buonavoglia, C., 2005. Canine parvovirus infection: Which diagnostic test for virus. J. Virol. Meth., 126, 179–185.

Esfandiari, J., Klingeborn, B., 2000. A comparative study of a new rapid and one-step test for the detection of parvovirus in faeces from dogs, cats and mink. J. Vet. Med., B. 47, 145–153.

Firoozjaii, A., Shoorijeh, A., Mohammadi, A., Tamadon, A., 2011. Characterization of Iranian isolates of canine parvovirus in fecal samples using polymerase chain reaction assay. Iran. J. Biotech., 9, 63–68.

Gill, M., Srinivas, J., Morozov, I., Smith, J., Anderson, C., Glover, S., Champ, D., Chu, H., 2004. Three-Year Duration of Immunity for Canine Distemper, Adenovirus, and Parvovirus after Vaccination with a Multivalent Canine Vaccine. Int. J. Appl. Res.Vet. Med., Vol. 2, No.4.

Goddard, A., Leisewitz A.L., 2010. Canine parvovirus. Vet. Clin. N Amer. S. Anim. Pract., 40, 1041–1053.

Greene, C.E., Schultz, R.D., 2006. Immunoprophylaxis. In: Infectious diseases of the dog and cat (Greene CE, ed). 3rd edition, Saunders Elsevier, St Louis, USA. pp: 1101–1118.

Jamshidi, S., Mohyedini, S., Rafati, S., Nikbakht, G.R., Malmasi, A., Taslimi, Y., Akbarein, H., 2013. Comparison of immunochromatographic rapid test with molecular method in diagnosis of canine parvovirus. Iran. J. Vet.Med., 7, 57–61.

Kapil, S., Cooper, E., Lamm, C., Murray, B., Rezabek, G., Johnston, L., Campell, G., Johnson, B., 2007. Canine parvovirus types 2c and 2b circulating in North American dogs in 2006 and 2007. J. Clin. Microbiol., 45, 4044–4047.

Mccaw, D.L., Hoskins, J.D., 2006. Canine viral enteritis. In: Infectious diseases of the dog and cat (Greene CE, ed). 3rd edition, Saunders Elsevier, St Louis, USA, pp: 63–70.

Mochizuki, M., San Gabriel, M.V., Nakatani, H., Yoshida, M., Harasawa, R., 1993. Comparison of polymerase chain reaction with virus isolation, haemagglutination assays for the detection of canine parvoviruses in faecal specimens. Res. Vet. Sci., 55, 60–63.

Pereira, C.A., Monezi, T.A., Mehnert, D.U., D'Angelo, M., Durigon, L.E., 2000. Molecular characterization of canine parvovirus in Brazil by polymerase chain reactionassay. Vet. Microbiol., 75, 127–133.

Pollock, R.V., 1982. Experimental canine parvovirus infection in dogs. Cornel. Vet. 72,103–119.

Prittie, J., 2004. Canine parvoviral enteritis: a review of diagnosis, management, and prevention. J. Vet. Emer. Crit. Care., 13, 167–176.

Saknimit, M., Inatsuki, I., Sugiyama, Y., Yagami, K., 1988. Virucidal efficacy of physico-chemical treatments against coronaviruses and parvoviruses of laboratory animals. Jikken Dobutsu., 37,341–345.

Sakulwira, K., Vanapongtipagorn, P., Theampoonlers, A., Oraveerakul, K., Poovorawan, Y., 2003. Prevalence of canine coronavirus and parvovirus infections in dogs with gastro- enteritis in Thailand. Vet. Med – Czech., 48,163–167.

Savigny, M.R., Macintire, D.K., 2010. Use of oseltamivir in the treatment of canine parvoviral enteritis. J. Vet. Emer. Crit. Care., 20, 132–142.

Siedek, E.M., Schmidt, H., Sture, G.H., Raue, R., 2011. Vaccination with canine parvovirus type 2 (CPV-2) protects against challenge with virulent CPV-2b and CPV-2c. Berl. Munch Tier. Woch., 124, 58–64.

Spibey, N., Greenwood, N.M., Sutton, D., Chalmers, W., Tarpey, I., 2008. Canine parvovirus type 2 vaccine protects against virulent challeng with type 2c virus. Vet. Microbil., 128, 48–55.

Steneroden, K.K., Hill, A.E., Salman, M.D., 2011. A needs-assessment and demographic survey of infection-control and disease awareness in western US animal shelters. Prev. Vet. Med., 98, 52–57.

Truyen, U., 2006. Evolution of canine parvovirus- A need for new vaccines. Vet. Microbiol., 117, 9–13.

Uwatoko, K., Sunairi, M., Nakajima, M., Yamaura, K., 1995. Rapid method utilizing the polymerase chain reaction for detection of canine parvovirus in feces of diarrheic dogs. Vet. Microbiol., 43, 315–323.

Zhao, Y., Lin, Y., Zeng, X., Lu, C., Hou, J. 2013 Genotyping and pathobiologic characterization of canine parvovirus circulating in Nanjing, China. Virol. J., 10, 272.


Full Text: PDF

Refbacks

  • There are currently no refbacks.