Open Access Open Access  Restricted Access Subscription Access
Cover Image

Serodiagnosis and molecular survey on letospiral abortions from sheep farms in Tabriz and surrounding areas

Parisa Froutani, Hossein Hamali, Raziallah Jafari Jozani, Gholamreza Abdollahpour, Katayon Nofouzi, Gholamreza Norsaadat

Abstract


Leptospirosis is presumed to be the most widespread zoonosis in the world. The purpose of this study was to determine of seroprevalence of leptospirosis among the aborted ewes and in the same time, detection of bacterial DNA in aborted fetal tissues by the PCR protocol. A total number of 70 aborted fetuses and related placentas were submitted to the large animal clinic at the University of Tabriz, from October 2010 to March 2011.Tissue samples were collected from several fetal organs including liver, brain, kidney, lung, spleen, heart, stomach fluid and placenta, then separately pulverized under liquid nitrogen and finally stored at -20°C until DNA extraction. DNA extraction from frozen tissues samples was performed using a commercial kit (AccuPrep Genomic DNA Extraction Kit, Bioneer, S. Korea) following the manufacturer’s instructions. Of 70 submissions, 6(8/57%) samples were diagnosed positive to the leptospira Interrogans by the PCR. In contrast 7(10%) out of 76 dam’s sera were diagnosed as positive to leptospirosis by the microscopic agglutination test (MAT). One out of 70 animals was seropositive to serovar canicula, two animals to serovar pomona, two animals to grippotyphosa, one animal to serovars: grippotyphosa, pomona, hardjo and one animal to serovars: grippotyphosa, pomona, icterohaemorrhagiae. However, statistical difference was not observed between two diagnostic techniques (P≥0/05). In conclusion, both pomona and grippotyphosa serovars induced abortions were determined to be more common leptospiral abortions in sheep flocks in Tabriz (north-west of Iran).

References


Abdollahpour, G., 2011. Six years serobacteriological study of leptospirosis in14 provinces of Iran, Proceedings of Bacterial Waterborne and Emerging Infectious Diseases in North Africa and the Middle East, Collaborative Research Opportunities in the Middle East and North Africa Conference January 31-February 3, 2011 Nicosia, Cyprus., Page 12.

Abdollahpour, G., Shafighi, T., ,Sattari, T.S., 2009. Serodiagnosis of leptospirosis in cattle in north of Iran, Gilan. Int. J. Vet. Res., 3 (1). 7-10.

Cole, J.R., Sulzer, C.R., Pursell, A.R., 1973. Improved microtechnique for the leptospiral microscopic agglutination test. Appl. Microbiol., 25. 976-980.

Ebrahimi, A., Alijani, L., Abdollahpour, G., 2003. Serological survey of human leptospirosis in tribal areas of Farsan and Koohrang cities. Iran. J. Med. Sci., Vol 28, No. 2, June 93-96

Everard, J.D., Everard, C.O.R., 1993. Leptospirosis in the Caribbean. Rev. Med. Microbiol., 4. 114–122.

Faine, S., Adler, B., Bolin, C., Perolat, P., (2 nd. Edn.)., 1999. Leptospira and Leptospirosis. Melbourne. Med., P, 272.

Genovez, M.E., Del Fava, C., Castro, V., Gotti, T.B., Dib, C.C., Pozzi, C.R., Arcaro, J.R.P., Miyashiro, S., Nassar, A.F.C., Cirillo, S.L., 2006. Leptospirosis outbreak in dairy cattle due to Leptospira spp. serovar Canicola: reproductive rates and serological profile after treatment with streptomycin sulfate. Arq. Inst. Biol., São Paulo., 73(4). 389-393.

Haji Hajikolaei, M.R., Ghorbanpour, M., Gharibi, D., abdollapour, G.R., 2007. Serologic study on leptospiral infection in sheep in Ahvaz, southwestern Iran. Iran. J. Veter. Res., Shiraz University, 8(40)21, 333-336.

Haji Hajikolaei M.R., Gorbanpour Najafabadi, M., Abdollahpour, G., 2005. Serological study of Leptospirosis in cattle in Ahvaz. J. Fac. Vet. Med. Uni., Tehran, 60(1), 7-14.

Hassanpour, A., Imandar, M., Abdollahpour, G.R., Mashayekhi, M., 2011. Seroprevalence of Leptospiral infection in ewes in Khoy-Iran. Adv. Env. Biol., 5(8), 2033-2038

Kingscote , B., 1985. Leptospirosis in Sheep in Western Canada. Can. Vet. J., 26(5), 164, 165-168.

Levett, P.N., 2001. Leptospirosis . Clin. Microbiol. Rev., 14. 296-326.

Radostitis, O.M., Gay, C.C., Hinchcliff, K.W., Constable, P.D., 2007. Veterinary medicine. 10th Edn. Saunders Co. London., pp, 963-984.

Ratnam, S., 1994. Leptospirosis: an Indian perspective. Indian J. Med. Microbiol., 12. 228–239.

Sakhaee, E., Abdollahpour, G., 2011. Detection of leptospiral antibodies by microscopic agglutination test in north-east of Iran. Asian Pacif. J. Trop. Biomed., 227-229.

Shivaraj., Venkatesha, M.D., Sanjukta, R., Sripad, K., Sanjeevkumar., Chandranaik, B.M., Reukaprasad, C., 2009. Leptospirosis in sheep and its diagnosis. Veter. World., 2(7), 263-264.

Srivastava, S.K., 2006. Prospects of developing leptospiral vaccines for animals. Ind. J. Med. Microb., 24(4). 331-336.

Tooloei, M. , Abdollapour, G.R., Karimi, H., Hasanpor, A., 2008. Prevalence of Serum Antibodies Against Six Leptospira Serovars in Sheep in Tabriz, Northwestern Iran. J. Anim. Veter. Adv., 7(4), 450-55.


Full Text: PDF

Refbacks

  • There are currently no refbacks.