Molecular characterization of potential allergenic molds (Aspergillus, Penicillium and Fusarium) in two buildings receiving the public in Abidjan – Côte d’Ivoire

Authors

  • M’boh Epi Reine Elisabeth N’Gou Institut Pasteur of Côte d’Ivoire (Biology of Immunity Unit, Molecular Biology Platform, Mycology Laboratory, Biochemistry Laboratory, Microbiology Laboratory).
  • David Coulibaly N’Golo Institut Pasteur of Côte d’Ivoire (Biology of Immunity Unit, Molecular Biology Platform, Mycology Laboratory, Biochemistry Laboratory, Microbiology Laboratory).
  • Chiaye Claire Antoinette Yapo-crezoit Institut Pasteur of Côte d’Ivoire (Biology of Immunity Unit, Molecular Biology Platform, Mycology Laboratory, Biochemistry Laboratory, Microbiology Laboratory).
  • Ama Valerie Bonouman Institut Pasteur of Côte d’Ivoire (Biology of Immunity Unit, Molecular Biology Platform, Mycology Laboratory, Biochemistry Laboratory, Microbiology Laboratory).
  • Solange Kakou Institut Pasteur of Côte d’Ivoire (Biology of Immunity Unit, Molecular Biology Platform, Mycology Laboratory, Biochemistry Laboratory, Microbiology Laboratory).
  • Ossey Bernard Yapo Central Environmental Laboratory, Ivorian Anti-Pollution Centre (CIAPOL).
  • Allico Joseph Djaman Université Félix Houphouët Boigny of Côte d’Ivoire (Biology and Health Laboratory), 22 BP 582 Abidjan 22.
  • Mireille Dosso Institut Pasteur of Côte d’Ivoire (Biology of Immunity Unit, Molecular Biology Platform, Mycology Laboratory, Biochemistry Laboratory, Microbiology Laboratory).

Keywords:

Allergy, Moulds, Aspergillus, Penicillium, Fusarium, PCR.

Abstract

Aspergillus, Penicillium and Fusarium species play an important role in environmental allergy. These molds are potential density in the indoor environment. This study aimed to characterize the strains Aspergillus, Penicillium and Fusarium using a molecular method. The beta-tubulin and elongation factor TEF- genes were utilized to differentiate the species. A total of 405 environmental species (Aspergillus, Penicillium and Fusarium) were isolated, purified and initially identified by colony morphology. Subsequently, DNA was extracted and; PCR was performed from 351 isolates. The results were then compared to morphological characteristics. Of the 351 isolates tested, 167 were Aspergillus, 103 were Penicillium and 81 were Fusarium. The beta-tubulin and elongation factor TEF genes were found to be the most suitable for differentiating these three genera among them; the beta-tubulin gene was used for molecular identification of Aspergillus, Penicillium and the elongation factor TEF gene for characterizing Fusarium species.

References

Abastabar, M., Mirhendi, H., Rezaei-Matehkolaei, A., Shidfar, M.R., Kordbacheh, P., Makimura, K., 2014. Restriction analysis of beta-tubulin gene for differentiation of the common pathogenic dermatophytes. J. Clin. Lab. Anal., 28, 91-96.

Bakar, A.A.I., Izzati, M.Z.N.A., Kalsom, U.Y., 2013. Diversity of Fusarium species associated with post-harvest fruit rot disease of tomato. Sains Malaysiana, 42, 911-920.

Balajee, S.A., Gribskov, J.L., Hanley, E., Nickle, D., Marr, K.A., 2005. Aspergillus lentulus sp. Nov. a new sibling species of A. fumigatus. Eukaryot Cell, 4, 625-632.

Bialek, R., Konrad, F., Kern, J., 2005. PCR based identification and discrimination of agents of mucormycosis and aspergillosis in paraffin wax embedded tissue. J. Clin. Pathol., 58, 1180-1184.

Bougnoux, M.E., Espinasse, F., 2003. Nouvelles applications des techniques de biologie moléculaire en mycologie médicale. Revue Française des Laboratoires, 351, 67-71.

Ceylan, E., Ozkutuk, A., Ergor, G., Yucesoy, M., Itil, O., Caymaz, S., 2006. Fungi and indoor conditions in asthma patients. J. Asthma, 43, 789-794.

El-Morsy, E.S.M., 2006. Preliminary survey of indoor and outdoor airborne microfungi at coastal buildings in Egypt. Aerobiol., 22, 197-210.

Frisvad, J.C., Samson, R.A., 2004. Polyphasic taxonomy of Penicillium subgenus Penicillium. A guide to identification of food and airborne terverticillate Penicillia and their mycotoxins. Stud. Mycol., 49, 174.

Geiser, D.M., Jiménez-Gasco, M.M., Kang, S., Makalowska, I., Veeraraghavan, N., Ward, T.J., Zhang, N., Kuldau, G.A., O’Donnell, K., 2004. FUSARIUM-ID v. 1.0: A DNA sequence database for identifying Fusarium. Eur. J. Plant Pathol., 110, 473-479.

Glass, N.L., Donaldson, C.G., 1995. American society for microbiology development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol., 61, 1323-1330.

Hong, S.B., Go, S.J., Shin, H.D., Frisvad, J.C., Samson, R.A., 2005. Polyphasic taxonomy of Aspergillus fumigatus and related species. Mycol., 97, 1316-1329.

Horner, W.E., Helbling, A., Salvaggio, J.E., Lehrer, S.B., 1995. Fungal allergens. Clin. Microbiol. Rev., 8, 161-179.

Houbraken, J., Samson, R.A., 2011. Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Stud. Mycol., 70, 1-51.

Husain, S., Kwak, E.J., Obman, A., 2004. Prospective assessment of Platelia Aspergillus galactomannan antigen for the diagnosis of invasive aspergillosis in lung transplant recipients. Am. J. Transplant., 4, 796-802.

Johnston, C.L., 2008. Identification of Penicillium species in the South African litchi export chain. Department of Microbiology and Plant Pathology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa, 123p.

Klich, M.A., 2002. Identification of common Aspergillus species. Utrecht, The Netherlands: Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands, 116p.

Kristensen, R., Torp, M., Kosiak, B., Holst-Jensen, A., 2005. Phylogeny and toxigenic potential is correlated in Fusarium species as revealed by partial translation elongation factor 1 alpha gene sequences. Mycol. Res., 109, 173-186.

Kvas, M., Marasas, W.F.O., Wingfield, B.D., Wingfield, M.J., Steenkamp, E.T., 2009. Diversity and evolution of Fusarium species in the Gibberella fujikuroi complex. Fungal Divers., 34, 1-21.

Laforgue, R., Guérin, L., Pernelle, J.J., Monet, C., Dupont, J., Bouix, M., 2009. Evaluation of PCR-DGGE methodology to monitor fungal communities on grapes. J. Appl. Microbiol., 107, 1208-1218.

N’Gou, M.E.R.E., Yapo-Crezoit, C.C.A., Bonouman, A.V., Kouao, D., Djaman, A.J., Dosso, M., 2021. Biodiversité des Moisissures - Abidjan - Côte d’Ivoire. Moulds Biodiversity in Abidjan - Côte d’Ivoire. Revue française d’allergologie. 61(5), 351-356.

NF EN ISO 16000-19 Novembre, 2014. Air intérieur - Partie 19: stratégie d'échantillonnage des moisissures.

NF ISO 16000-21 Février, 2014. Air intérieur-Partie 21: détection et dénombrement des moisissures - Échantillonnage à partir de matériaux.

O’Donnell, K., Kistler, H.C., Cigelnik, E., Ploetz, R.C., 1998. Applied biological sciences multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies. Proc. Natl. Acad. Sci. USA, 95, 2044-2049.

Oliveira, M., Ribeiro, H., Abreu, I., 2005. Annual variation of fungal spores in atmosphere of Porto: 2003. Ann. Agr. Environ. Med., 12, 309-315.

Ozkara, A., Ocak, I., Korcan, S.E., Konuk, M., 2007. Determination of fungal air spora in Afyonkarahisar, Turkey. Mycotaxon, 102, 199-202.

Pasqualetti, M., Giovannini, V., Barghini, P., Gorrasi, S., Fenice, M., 2020. Diversity and ecology of culturable marine fungi associated with Posidonia oceanica leaves and their epiphytic algae Dictyota dichotoma and Sphaerococcus coronopifolius. Fungal Ecol., 44, 100906.

Pei- Chih, W., Huey-Jen, S., Chia-Yin, L., 2000. Characteristic of indoor and outdoor airborne fungi at suburban and urban homes in two Season. Sci. Total Environ., 253, 111-118.

Pepeljnjak, S., Segvic, M., 2003. Occurrence of fungi in air and on plants investigation of different climatic regions in Croatia. Aerobiol., 19, 11-19.

Plewa-Tutaj, K., Lonc, E., 2014. Molecular identification and biodiversity of potential allergenic molds (Aspergillus and Penicilium) in the poultry house: first report. Aerobiol., 30, 445-451.

Samson, R.A., Houbraken, J., Thrane, U., Frisvad, J.C., Andersen, B., 2010. Food and indoor fungi. Centraalbureau voor Schimmelcultures (CBS-KNAW Fungal Biodiversity Centre: Utrecht, The Netherlands). 390p.

Sanchez Espinosa, K.C., Almaguer Chavez, M., Duarte-Escalante, E., Rojas Flores, T.I., Frias-De-Leon, M.G., Reyes-Montes, M.D.R., 2021. Phylogenetic identification, diversity, and richness of aspergillus from homes in Havana, Cuba. Microorganisms, 9, E115.

Shokouhi, G.R., Mirhendi, S.H., Kordbachcheh, P., Nikaein, M., Matehekolaei, A.R., Abbastabar, M., 2011. Morphological and genotypic identification of some environmental isolates of Aspergillus in Iran based on beta-tubulin gene sequencing. J. Isfahan Med. Sch., 166, 1-10.

Simon-Nobbe, B., Denk, U., Pöll, V., Rid, R., Breitenbach, M., 2008. The spectrum of fungal allergy. Int. Arch. Allergy Immunol., 145, 58-86.

Tsui, K.M., Hyde, K.D., Hodgkiss, I.J., 2000. Biodiversity of fungi on submerged wood in Hong Kong streams. Aquat. Microb. Ecol., 21, 289-298.

Twaroch, T.E., Curin, M., Valenta, R., Swoboda, I., 2015. Mold allergens in respiratory allergy: From structure to therapy. Allergy Asthma Immunol. Res., 7, 205-220.

Verscheure, M., Lognay, G., Marlier, M., 2002. Revue bibliographique: les méthodes chimiques d’identification et de classification des champignons. Biotechnol. Agron. Soc. Environ., 6, 131-142.

Yaguchi, T., Horie, Y., Tanaka, R., Matsuzawa, T., Ito, J., Nishimura, K., 2007. Molecular phylogenetics of multiple genes on Aspergillus section Fumigati isolated from clinical specimens in Japan. Nippon Ishinkin Gakkai Zasshi. 48, 37-46.

Published

2021-03-29

How to Cite

N’Gou, M. E. R. E. ., N’Golo, D. C. ., Yapo-crezoit, C. C. A. ., Bonouman, A. V. ., Kakou, S. ., Yapo, O. B. ., Djaman, A. J. ., & Dosso, M. . (2021). Molecular characterization of potential allergenic molds (Aspergillus, Penicillium and Fusarium) in two buildings receiving the public in Abidjan – Côte d’Ivoire. Scientific Journal of Review, 7(2), 181-187. Retrieved from http://sjournals.com/index.php/sjr/article/view/1651

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Original Article