Quantification of cytokines in the amniotic fluid at the moment of delivery of Nelore calves conceived by in-vitro production and artificial insemination

Authors

  • C.F. Moya-Araujo School of Veterinary Medicine – FIO, Ourinhos/SP, Brazil
  • N. C. Prestes School of Veterinary Medicine and Animal Science – Sao Paulo State University – UNESP, Botucatu/SP – Brazil
  • M. Piagentini School of Veterinary Medicine and Animal Science – Sao Paulo State University – UNESP, Botucatu/SP – Brazil
  • G.H. M. Araujo School of Veterinary Medicine – FIO, Ourinhos/SP, Brazil
  • A.C. Camplesi School of Agriculture and Veterinary Science – Sao Paulo State University – UNESP, Jaboticabal/SP, Brazil
  • M. G. Silva School of Veterinary Medicine and Animal Science – Sao Paulo State University – UNESP, Botucatu/SP – Brazil

Keywords:

Nelore calves, amniotic fluid, in-vitro production, artificial insemination, cytokines

Abstract

The aim of the present study was to compare the concentrations of cytokines in the conceptus’ amnioticfluid at the moment of delivery of Nelore calvesconceived from embryos produced by in-vitro(IVP) or after artificial insemination (AI). Forty cows were divided into 2groups: 1- 20 cross-bred Nelore cows pregnant with Nelore calves derived from IVP embryos produced from oocitum pick up (OPU) oocytes; 2 - 20 pregnant Nelore cows bearing AI Nelore calves.The animals were fed pasture and had a free access to mineral salt and water.Around labor, cows were transferred to a maternal paddock permitting deliveryobservation. During the expulsion phase of the delivery, the amnion waspunctured by 40x12g needle and a 20 mL syringe and the fluid was collected intoa sterile plastic tube and stored in a freezer at -20C.

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Published

2014-02-25

How to Cite

Moya-Araujo, C. ., C. Prestes, N., Piagentini, M. ., M. Araujo, . G., Camplesi, A. . ., & G. Silva, M. (2014). Quantification of cytokines in the amniotic fluid at the moment of delivery of Nelore calves conceived by in-vitro production and artificial insemination. Scientific Journal of Medical Science, 3(2), 49-52. Retrieved from http://sjournals.com/index.php/sjms/article/view/843

Issue

Vol. 3 No. 2 (2014): February

Section

Short Communication

License

Copyright (c) 2014 C.F. Moya-Araujo, N. C. Prestes, M. Piagentini, G.H. M. Araujo, A.C. Camplesi, M. G. Silva

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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