In-vitro evaluation of fungicides, bioagents and aqueous leaf extracts against Alternaria leaf blight of cotton

Authors

  • V. M. Gholve Department of Plant Pathology College of Agriculture,Marathwada Krishi Vidyapeeth, Parbhani – 431 402 (MS)
  • S. M. Jogdand Department of Plant Pathology College of Agriculture,Marathwada Krishi Vidyapeeth, Parbhani – 431 402 (MS)
  • G. P. Jagtap Department of Plant Pathology College of Agriculture,Marathwada Krishi Vidyapeeth, Parbhani – 431 402 (MS)
  • U. Dey Department of Plant Pathology College of Agriculture,Marathwada Krishi Vidyapeeth, Parbhani – 431 402 (MS)

Keywords:

Gossypium spp., Alternaria macrospora, Fungicide, Bioagents, Aqueous leaf extracts, Mycelial inhibition

Abstract

All the six fungicides viz.,Mancozeb (75% WP); Carbendazim (50WP), Copper oxychloride (50WP), Captan (50 WP), Thiram (75 % WP), Chlorothalonil (75 WP) evaluated in vitro (@500, 1000 and 1500 ppm) were found effective against A. macrospora and caused significant inhibition of test pathogen over untreated control. However, Thiram was found most effective and recorded significantly highest mean mycelial inhibition (90.42%). This was followed by the fungicides, Captan (82.04%), Mancozeb (79.88%), Carbendazim(77.5%), Chlorothalonil (74.52%) and copper oxychloride (71.75%). All the five fungal and one bacterial bioagents/ antagonists evaluated in vitro against A. macrospora were found antifungal/ antagonistic against the test pathogen. However, T. viride was found most effective and recorded least linear mycelial growth (32.72 mm) with corresponding significantly highest mycelial inhibition (63.64%) of the test pathogen. The second and third best bioagents found were T. koningii and P. fluorescens, which recorded linear mycelial growth, respectively of 33.90 mm and 33.95 mm with corresponding mycelial inhibition, respectively of 62.33 and 62.27 per cent. All the six botanicals/plant extracts evaluated in vitro (@5, 10 and 15 % each) were found fungistatic/ fungicidal against. A.macrospora. However, significantly least mean radial mycelial growth (56.18mm) and significantly highest mean mycelial inhibition (37.47%) was recorded with Garlic. The second and third best botanicals found were onion and Tulsi which recorded second and third least mean radial mycelial growth, respectively of 58.52 mm and 62.51 mm with corresponding mean mycelial inhibition of 34.97 and 32.86 per cent, respectively.

References

Anonymous, 2010. Cotton advisory board, Ministry of Agriculture, Government of India.

Barnwal, G.K., Jha, D.K., Dubey, S.C., 1998. Evaluation of different plant extracts against blight of marigold.

Phytopath. 10(2), 221-223.

Bashi, E., Sachs, Y., Rotem, J. 1983. Relation between disease and yield in cotton field affected by Alternaria

macrospora. Phytoparasitica. 11, 89-97.

Bhaskaran, R., Shanmugam, N., 1973. Laboratory evaluation of some fungicides against Alternaria macrospora

Zimm. Madras Agric. J. 60 (7), 646-647.

Bhattiprolu, S.L., Prasada Rao, M.P., 2009. Management of Alternaria leaf spot (Alternaria macrospora zimm.) on

cotton by biological approach. J. Cotton Res. Devp. 23(1), 135-137.

Chauhan ,M.S., Yadav, J.P.S., Benniwal, J., 1997. Field resistance of cotton to Myrothecium leaf spot and Alternaria

leaf spot (A.macrospora) diseases. J. Cotton Res. Dev. 11, 196-205.

Chidambaram, P.A., Kanpan, N., Krishna, G., Johnson, J., Kumar, A., 2002. Chemical and biological management of

cotton foliar diseases and biochemistry of their interactions in cotton (Abst). In : Symposium on ‘Major Plant

Diseases and Their Management, held at CICR, Nagpur. Pp, 27.

Dalpati, N.N.S., Parate, R.L., Ingle, S.T., 2010. Efficacy of some bioagent’s and botanical’s against Alternaria

macrospora causing leaf spot of cotton. J. Pl. Dis. sci. 5(1), 95-97.

Gangurde, P.B.; S.R. Zanjare; S.S.Mehetre; S.B. Rajmane and C.B. Wayal. (2003). In vitro evaluation of chemicals

and bioagents against Alternaria macrospora Zimm. causing leaf spot of cotton. J. Cotton Res. Devp. 17(2) :

-265.

Gupta, S.S., Prasad, V., 1968. Inhibitory effect of some fungicides on the production and activity of pectic enzymes

of Alternaria tenuis. Rgrk. Agra. Univ. J. 17, 161-165.

Karade, V.M., Sawant, D.M., 1999. Effect of some plant extracts on the spore germination of Alternaria

alternate.Pl. Dis. Res. 14(1), 75-77.

Lokesh, M.S., Hiremath, R.V., 1988. Antagonism of Trichoderma spp. against seed mycoflora of red gram. Pl. Path.

Newsletter. 6(1-2), 31-32

Mayee C.D., Mukewar, P.A., 2007.Loss-inducing diseases of cotton and their management.In: Cotton in Andhra

Pradesh (eds:Rao,NGP,Appa Rao and Siddique E.A. ).Farm and Rural Science Foundation and ANGRAU,

Hydrabad. Pp,348.

Mehetre, S.S., Zanjare, S.R., Sawashe, S.G., Rajmane, S.B., Wayal, C.B., 2003. Biological control of Alternaria leaf

blight of cotton caused by Alternaria macrospora. J. Cotto. Res. Devp. 17(2), 208-210.

Naik, U.R., fugro, P.A., Kadam, J.J., Jadhav, D.K., 2010. Exploration of fungicides, bioagent’s and botanical’s against

leaf blight of okra incited by Alternaria chlamydospora. J. Pl. Dis. Sci. 5(1),37-40.

Nene,Y.L., Thapliyal, P.N., 1993. Evolution of fungicides In: Fungicides in Plant Disease Control (3rd ed.). Oxford, IBH

Pub.Co., New Delhi. Pp, 331.

Padmanabhan, P, Naryanaswamy, P., 1976. Fungicidal control of leaf spot disease of cotton caused by Alternaria

macrospora. Madras Agric. J. 63 (4), 271-272.

Padule, D.N., Shinde, P.A., 1989. Efficacy of fungicides against Alternaria blight of cotton. J. Maharashtra Agril.

Univ. 14(1), 122-123.

Palazon, I.J., Palazon, C.F., 1988. Study of the antagonism between Trichoderma viride and different species of

fungi causing pomegranate fruit rots.Lannga Tenerife Spain Centro de Investigcion 7 technologia Agarias. 273-

Ramegowda, G., Naik, M.K., Sunkad, G., Patil, M.B., Sataraddi, A., 2007. Evaluation of fungicides, botanicals and

bioagents against leaf spot caused by Alternaria macrospora in cotton. Indian J. Pl. Prot. 35(2), 130-134.

Rane, M.S., Patel, M.K., 1956. Diseases of Cotton in Bombay: Alternaria leaf spot of cotton. Indian Phytopath. 9,

-113.

Shravelle, E.G., 1961. The nature and use of modern fungicides.Burgers Publ.co., Minneapolics, Minnesota, USA,

PP, 308.

Singanmathi, B.V., Ekbote, M.V., 1960. The chemical control of Alternaria leaf blight of cotton in Maharashtra

state. J. Maharashtra Agric. Univ. 5, 87-88.

Srinivasan, K.V., 1994.cotton diseases. Indian Society for Cotton Improvement.Bombay. pp, 156-164.

Uppal, B.N., Patel, M.K., Kamat, M.M., 1935. The fungi of Bombay Dept. of Agric. Bull. No. 176.28.

Vasudeva, R.S., 1960. Diseases of cotton in India: A monograph (Dastur, R.H., Asana, R.D., Sawhney, K., Sikka, S.M.,

Vasudeva, R.S., Khan, Q., Rao, V.P., Sethi, B.L., eds.) 2, 164-216.

Woo, S.L., Scala, F., Ruocco, M., Lorito, M., 2005. The molecular biology of interaction between Trichoderma sp.

and phytopathogenic fungi and plants. Phytopath. 96(2), 181-185.

Published

2012-07-06

How to Cite

M. Gholve, V. ., M. Jogdand, S. ., P. Jagtap, G. ., & Dey, U. . (2012). In-vitro evaluation of fungicides, bioagents and aqueous leaf extracts against Alternaria leaf blight of cotton. Scientific Journal of Crop Science, 1(1), 12-21. Retrieved from http://sjournals.com/index.php/sjcs/article/view/1502

Issue

Section

Original Article