Cover Image

Antimicrobial effect of aqueous and ethanolic extracts Teucrium polium L. on Streptococcus pyogenes, Pseudomonas aeruginosa and Staphylococcus epidermidis

Farideh Tabatabaei Yazdi, Behrooz Alizadeh Behbahani

Abstract


Teucrium polium L. (Labiatae) has long been recognized in folk medicine in the treatment of many pathophysiological implications, such as gastrointestinal disorders, inflammations, diabetes and rheumatism. In this study antimicrobial activity of two crude extracts obtained from Teucrium polium L. was tested against bacterial species. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using a microdilution analysis method. The antimicrobial effects of extracts were evaluated on Streptococcus pyogenes PTCC 1447, Pseudomonas aeruginosa PTCC 1310 and Staphylococcus epidermidis PTCC 1435 by “using the method of Collins” and “disk agar diffusion method”. The results showed that aqueous and ethanolic extracts were quite effective in 2000 μg/ml concentration on Streptococcus pyogenes and Staphylococcus epidermidis and were prevented from growth them on medium, while both extracts have no certain antimicrobial effect on Pseudomonas aeruginosa. In “disk agar diffusion method”, 10, 20, 30 and 40 mg/ml aqueous and alcoholic extracts concentrations, was inhibition effect on Streptococcus pyogenes and Staphylococcus epidermidis, and 30 and 40 mg/ml aqueous and ethanolic extracts concentrations, has inhibition effect on Pseudomonas aeruginosa prevent them growing, but at 10 and 20 mg/ml concentrations, no inhibitory effect on Pseudomonas aeruginosa was observed. The results indicate that ethanolic and aqueous extracts of Teucrium polium L. have the greatest effect on gram-positive bacterium Streptococcus pyogenes (p<0.05). Results showed, aqueous and ethanolic extracts of Teucrium polium L., have been strong antimicrobial activity against many food pathogen bacteria.


References


Ahmad, I., Beg, A.Z., 2001. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogen. J. Ethnopharmacol., 74, 113-123.

Akin, M., Oguz, D., Saracoglu, H., 2010. Antibacterial Activity of Essential oil from Thymbra spicata var. spicata L. and Teucrium polium (Stapf Brig.). Interventions., 8 (9), 53-58.

Alizadeh Behbahani, B., Tabatabaei Yazdi, F., Shahidi, F., Mohebbi, M., 2012. Antimicrobial activity of Avicennia marina extracts ethanol, methanol & glycerin against Penicillium digitatum (citrus green mold). Scient. J. Micro., 1(7), 147-151.

Babayi, H., Kolo, I., Okogun, J., Ijah, U., 2004. The antimicrobial activities of methanolic extracts of Eucalyptus camaldulensis and Terminaliacatappa against some pathogenic microorganisms. Biokemistri. 16(2); 106-111.

Barbour EK, Al Sharif M, Sagherian VK, Habre AN, Talhouk RS, Talhouk SN. 2004. Screening of selected indigenous plants of Lebanon for antimicrobial activity. J. Ethnopharm., 93, 1-7.

Bauer, A., Kirby, W., Sherris, J.C., Turck, M., 1966. Antibiotic susceptibility testing by a standardized single disk method. American J. Clin. Path., 45(4), 493-496.

Benger, S., Townsend, P., Ashford, R.L., Lambert, P., 2004. An in vitro study to determine the minimum inhibitory concentration of Melaleuca alternifolia against the dermatophyte Trichophyton rubrum. The Foot., 14, 86 - 91.

Collins, C.H., Lynes, P.M., Grange, J.M., 1995. Microbiological Methods. (7thEdn.) Butterwort-Heinemann Ltd., Britain, pp. 175-190.

Cowan, M., 1999. Plant Products as antimicrobial agents, Clinical Microbiology Reviews, 12, 546-582.

Darabpour, E., Motamedi, H., Seyyed Nejad, S.M., 2010. Antimicrobial properties of Teucrium polium against some clinical pathogens. Asian Paci. J. Tropic. Med.

Espinel-Ingroff, A., Fothergill, A., Peter, J., Rinaldi, M., Walsh, T., 2002. Testing conditions for determination of minimum fungicidal concentrations of new and established antifungal agents for Aspergillus spp.: NCCLS collaborative study. J. Clin. Micro., 40(9), 3204-3208.

Ghalem, B.R., Mohamed, B., 2008. Antibacterial activity of leaf essential oils of Eucalyptus globulus and Eucalyptus camaldulensis. African J. Pharm. Pharmaco., 2(10), 211-215.

Khan, R., Islam, B., Akram, M., 2009. Antimicrobial activity of five herbal extracts against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin. Molecules., 14, 586-597.

Kizil, S., Sogut, T., 2003. Investigation of Antibacterial Effects of Some Spices. Crop Res., 25(1), 86-90.

Mahmoud, M., Al-Ameri, S.A., Abbas, S., 2011. Extraction, Identification and Antimicrobial Activity of Some Phenolic Acids as Antioxidants in Teucrium polium Plant. Kerbala J. Pharma. Sci., 2, 170-188.

Manandhar, N.P., 1995. A survey of medicinal plants of Jajarkot district Nepal, J. Ethnopharmacol., 48, 1-6.

Pauli, A., Knobloch, K., 1987. Inhibitory effects of essential oil components on growth of food-contaminating fungi. Zeitschrift für Lebensmitteluntersuchung und-Forschung A, 185(1), 10-13.

Ricci, D., Fraternale, D., Giamperi, L., 2005. Chemical composition, antimicrobial and antioxidant activity of the essential oil of Teucrium marum (Lamiaceae). J. Ethnopharm., 98, 195-200.

Sarac, N., Ugur, A., 2007. Antimicrobial activities and usage in folkloric medicine of some Lamiaceae species growing in Mugla, Turkey. EurAsia. J. Bio. Sci. 4, 28-37.

Tassou, C.C., Nychas, G.J., 1995. Antimicrobial activity of the essential oil of Mastic fum on gram positive and gram negative bacteria in broth and model food systems. Int. Biodeterio. biodegrad. 36, 411- 20.

Valero, M., Salmeron, M., 2003. Antimicrobial activity of 11 essential oils against Bacillus cereus in Tyndallized carrot broth. Int. J. Food Micro., 8, 73-81.

Yazdani, D.R.S., Amin, Gh., Zainal Abidin, M.A. ,Shahnazi, S., Jamalifar, H., 2009. Antifungal Activity of Dried Extracts of Anise (Pimpinella anisum L.) and Star anise (Illicium verum Hook. f.) Against Dermatophyte and Saprophyte Fungi. J. Med. Plant., 8(5), 24-9.


Full Text: PDF

Refbacks

  • There are currently no refbacks.