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Time course for cellulase enzyme production by Aspergillus Flavus using different organic nitrogen sources

I.F. Okonkwo, F.J.C. Odibo, C.M. Obele


The time course for the production of cellulase enzyme of Aspergillus flavus was carried out at 35οC in a shaken incubator using modified Mandel and Weber’s medium containing in ( g/l) (NH4)2S0­4 1.4, KH2P04 2.0, Urea 0.3, MgS047H20 0.3, CaCl3 0.3 FeS047H20 0.005, ZnS047H200.0014, MnS04H20 0.0016, CoCl3 0.002, Tween 80  2.0 ml, Carboxymethylcellulose 10.0 and pH 6.8. It indicated a period of 120 h (5days) as the optimum incubation period under the experimental condition using CMC as substrate.  The mycelia growth pattern (biomass) indicated highest growth on the fifth day also corresponding to the time of maximum enzyme activity.  The results from the studies also showed that Mandel and Weber’s Medium was better than other substitutions with alternative nitrogen sources in all the assays, followed by yeast extract and the least being soya bean meal. The study therefore maintained that Mandel and Weber’s Medium is the best at 5 days incubation for cellulase production by Aspergillus flavus.


Adsul, M.G., Bastawde, K.B., Varma, A.J., Gokhale, D.V., 2007. Strain improvement of Penicillium janthinellum NCIM 1171 for increased cellulase production.Bioresour. Technol., 98, 1467-1473.

Bradford, M.M., 1976. A rapid and sensitive method, for quantification of microgram quantities of proteins, utilizing the principle of protein dye binding. Anal. Biochem., 72, 248-254.

Dedavid, E.S.L.A., Lopes, F.C., Silveira, S.T., Brandelli, A., 2008. Production of cellulolytic enzymes by Aspergillus phoenicis in grape waste using response surface methodol. Appl. Biochem. Biotechnol., DOI 10.1007/s12010-008-8190-7.

Kang, S.W., Park, Y.S., Lee, J.S., Hong, S.I., Kim, S.W., 2004. Produciton of cellulases andhemicellulases by Aspergillus niger KK2 from lignocellulosic biomass. Bioresour. Technol., 153-156.

Kirchner, O.G., Granados, M.S., Pascual, P.R., 2005. Effect of media composition and growth conditions on production of β-glucosidase by Aspergillus niger C-6. Appl. Biochem. Biotechnol., 121, 347-359

Lockington, R.A., Rodbourn, L., Barnett, S., Carter, C.J., Kelly, J.M., 2002. Regulation by carbon and nitrogen sources of a family of cellulases in Aspergillus nidulans. Fung. Gen. Biol., 37, 190-196.

Mandels, M., Weber, J., 1969. The Production of cellulases, In, Hajny G.J., Reese, E.T (Eds), cellulases and their applications, Am. Chem Soc., Washington D. C. pp 319-414.

Miller, G.L., 1959), Use of dinitrossalicylic acid reagent for determination of reducing sugars. Anal. Chem., 31, 426-428.

NIMET (2006); Nigerian meterological Agency, Onitsha, Anambra.

Ogel, Z.B., K. Yarangumeli, H. Du and J. Ifrij (2001), Submerged cultivation of Scytalidium thermophilum on complex lignocellulosic biomass. Enzyme Micobiol. Technol., 28, 689-695.

Ojumu, T., Solomon, V., Bamidele, O., Betiku, E., Layokun, S.K., Amigun. B., 2003. Cellulase production by Aspergillus flavus Linn Isolate NSPR 101 fermented in sawdust, bagass and corncob, African J. Biotechnol., 2 (6), 150–152.

Ong, L.G.A., Abd-Aziz, S., Noraini, S., Karim, M.I.A., Hassan, M.A., 2004. Enzyme production and profile by Aspergillus niger during solid Substrate fermentation using palm kernel cake as substrate. Appl.Biochem. Biotechnol., 118, 73-79.

Stephen, R.D., William, S.A., Edward, J., Todd, B.V., Michael, E.H., 2003. Automated Filter paper assay for determination of cellulose activity. Appl. Biochem. Biotech., 108, 689-703.

Wang, X.J., Bai, J.G., Liang, Y.X., 2006. Optimization of multienzyme production by two mixed strains in solid-state fermentation. Appl. Microbiol. Bbiotechnol., 73, 533-540.

Zhou, J., Wang, Y.H., Chu, J., Zhuang, Y.P., Zhuang, S.L., Yin, P., 2008. Identification and purification of the main components of cellulases from a mutant strain of Trichoderma viride T 100-14. Bioresour. Technol., 99, 6826-6833.

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