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Time course for cellulase enzyme production by Aspergillus Flavus using different organic nitrogen sources

I.F. Okonkwo, F.J.C. Odibo, C.M. Obele

Abstract


The time course for the production of cellulase enzyme of Aspergillus flavus was carried out at 35οC in a shaken incubator using modified Mandel and Weber’s medium containing in ( g/l) (NH4)2S0­4 1.4, KH2P04 2.0, Urea 0.3, MgS047H20 0.3, CaCl3 0.3 FeS047H20 0.005, ZnS047H200.0014, MnS04H20 0.0016, CoCl3 0.002, Tween 80  2.0 ml, Carboxymethylcellulose 10.0 and pH 6.8. It indicated a period of 120 h (5days) as the optimum incubation period under the experimental condition using CMC as substrate.  The mycelia growth pattern (biomass) indicated highest growth on the fifth day also corresponding to the time of maximum enzyme activity.  The results from the studies also showed that Mandel and Weber’s Medium was better than other substitutions with alternative nitrogen sources in all the assays, followed by yeast extract and the least being soya bean meal. The study therefore maintained that Mandel and Weber’s Medium is the best at 5 days incubation for cellulase production by Aspergillus flavus.

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